The potential for complementary targeted/non-targeted screening of novel psychoactive substances in equine urine using liquid chromatography-high resolution accurate mass spectrometry
Research output: Contribution to journal › Journal article › Research › peer-review
Standard
The potential for complementary targeted/non-targeted screening of novel psychoactive substances in equine urine using liquid chromatography-high resolution accurate mass spectrometry. / Cawley, Adam; Pasin, Daniel; Ganbat, Namuun; Ennis, Laura; Smart, Corrine; Greer, Candace; Keledjian, John; Fu, Shanlin; Chen, Alex.
In: Analytical Methods, Vol. 8, No. 8, 28.02.2016, p. 1789-1797.Research output: Contribution to journal › Journal article › Research › peer-review
Harvard
APA
Vancouver
Author
Bibtex
}
RIS
TY - JOUR
T1 - The potential for complementary targeted/non-targeted screening of novel psychoactive substances in equine urine using liquid chromatography-high resolution accurate mass spectrometry
AU - Cawley, Adam
AU - Pasin, Daniel
AU - Ganbat, Namuun
AU - Ennis, Laura
AU - Smart, Corrine
AU - Greer, Candace
AU - Keledjian, John
AU - Fu, Shanlin
AU - Chen, Alex
PY - 2016/2/28
Y1 - 2016/2/28
N2 - The potential for liquid chromatography-high resolution accurate mass (LC-HRAM) spectrometry to identify 'unknown' compounds using non-targeted screening methods provides a potential advantage in the fight against doping in sport. This innovation comes with the requirement for assessment to support its use in the medico-legal context. A method for the LC-HRAM detection of 2,5-dimethoxy-N-(2-methoxybenzyl)phenethylamine (NBOMe) compounds in equine urine was validated in order to assess the capabilities of a workflow developed for non-targeted analysis using the SIEVE® differential analysis software platform. Six NBOMe compounds (25B, 25C, 25D, 25E, 25H and 25I) were studied to develop and optimize the proposed non-targeted screening workflow before two additional candidates (25N and 25T2) were used as blind controls for verification. Chromatographic alignment and the integration threshold were found to be the most critical parameters for successful identification of 'unknown' responses. The proposed workflow serves as an example for anti-doping laboratories to implement fit-for-purpose non-targeted screening methods.
AB - The potential for liquid chromatography-high resolution accurate mass (LC-HRAM) spectrometry to identify 'unknown' compounds using non-targeted screening methods provides a potential advantage in the fight against doping in sport. This innovation comes with the requirement for assessment to support its use in the medico-legal context. A method for the LC-HRAM detection of 2,5-dimethoxy-N-(2-methoxybenzyl)phenethylamine (NBOMe) compounds in equine urine was validated in order to assess the capabilities of a workflow developed for non-targeted analysis using the SIEVE® differential analysis software platform. Six NBOMe compounds (25B, 25C, 25D, 25E, 25H and 25I) were studied to develop and optimize the proposed non-targeted screening workflow before two additional candidates (25N and 25T2) were used as blind controls for verification. Chromatographic alignment and the integration threshold were found to be the most critical parameters for successful identification of 'unknown' responses. The proposed workflow serves as an example for anti-doping laboratories to implement fit-for-purpose non-targeted screening methods.
UR - http://www.scopus.com/inward/record.url?scp=84958948781&partnerID=8YFLogxK
U2 - 10.1039/c6ay00156d
DO - 10.1039/c6ay00156d
M3 - Journal article
AN - SCOPUS:84958948781
VL - 8
SP - 1789
EP - 1797
JO - Analytical Methods
JF - Analytical Methods
SN - 1759-9660
IS - 8
ER -
ID: 239259358