The major diversification of Vγ1.1+ and Vγ2+ thymocytes in mice occurs after commitment to the γδ T-cell lineage
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The major diversification of Vγ1.1+ and Vγ2+ thymocytes in mice occurs after commitment to the γδ T-cell lineage. / Buus, Terkild B.; Geisler, Carsten; Lauritsen, Jens Peter H.
In: European Journal of Immunology, Vol. 46, No. 10, 11.10.2016, p. 2363-2375.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - The major diversification of Vγ1.1+ and Vγ2+ thymocytes in mice occurs after commitment to the γδ T-cell lineage
AU - Buus, Terkild B.
AU - Geisler, Carsten
AU - Lauritsen, Jens Peter H
PY - 2016/10/11
Y1 - 2016/10/11
N2 - γδ T cells are a heterogeneous cell population with different subsets playing specialized and often opposing roles during immune responses. A key question is whether γδ thymocytes are determined for their effector function already at an early stage, before their commitment to the γδ T-cell lineage, or are instructed during their later development. Here, we show that the adult Vγ1.1+ and Vγ2+ γδ T-cell subsets both go through a CD73+CD24+ development stage, and that the gene regulation involved in lineage commitment is shared by both subsets. We demonstrate that the major subset diversification first occurs after the cells have committed to the γδ T-cell lineage, strongly supporting an instructive model for functional programming of γδ T cells. In conclusion, we show that the two major adult γδ T-cell subsets in mice develop through a shared pathway utilizing similar cellular machinery and that they diverge after the CD24+CD73+ maturity stage.
AB - γδ T cells are a heterogeneous cell population with different subsets playing specialized and often opposing roles during immune responses. A key question is whether γδ thymocytes are determined for their effector function already at an early stage, before their commitment to the γδ T-cell lineage, or are instructed during their later development. Here, we show that the adult Vγ1.1+ and Vγ2+ γδ T-cell subsets both go through a CD73+CD24+ development stage, and that the gene regulation involved in lineage commitment is shared by both subsets. We demonstrate that the major subset diversification first occurs after the cells have committed to the γδ T-cell lineage, strongly supporting an instructive model for functional programming of γδ T cells. In conclusion, we show that the two major adult γδ T-cell subsets in mice develop through a shared pathway utilizing similar cellular machinery and that they diverge after the CD24+CD73+ maturity stage.
KW - CD73
KW - Cell differentiation
KW - Effector programming
KW - T-cell development
KW - γδ T cells
U2 - 10.1002/eji.201646407
DO - 10.1002/eji.201646407
M3 - Journal article
C2 - 27418188
AN - SCOPUS:84990862125
VL - 46
SP - 2363
EP - 2375
JO - European Journal of Immunology
JF - European Journal of Immunology
SN - 0014-2980
IS - 10
ER -
ID: 169080086