The effects of duodenal peptides on glucagon-like peptide-1 secretion from the ileum. A duodeno--ileal loop?
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Secretion of the gut hormone glucagon-like peptide-1 (GLP-1) is stimulated by meal ingestion. The response is rapid, suggesting a stimulatory pathway elicited from the upper gastrointestinal area. In pigs, we have been unable to demonstrate a neural stimulatory pathway, but GLP-1 secretion is regulated by local somatostatin secretion. In search for an endocrine pathway, we studied the effect of a range of concentrations of cholecystokinin octapeptide (26-33) (CCK 8), gastric inhibitory peptide 1-42 (GIP), secretin, motilin, calcitonin gene-related peptide (CGRP), and the modified amino acid, 5-hydroxytryptamine (serotonin, 5-HT) on GLP-1 and somatostatin release from isolated perfused segments of porcine ileum.GLP-1 secretion was stimulated by 1 nM CCK 8 and 10 nM GIP, but suppressed by 1 nM motilin and 1 microM 5-HT. Secretin and CGRP had no effect. Somatostatin secretion was stimulated by CCK 8 at 1 and 10 nM, by GIP at 1 and 10 nM and by 10 nM CGRP. Secretin, 5-HT and motilin had no effect on somatostatin secretion. We conclude that CCK 8 and GIP 1-42 stimulated GLP-1 secretion, but only in concentrations greatly exceeding normal postprandial concentrations. Thus, we find it unlikely that endocrine agents from the duodenum regulate GLP-1 secretion in pigs.
Original language | English |
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Journal | Regulatory Peptides |
Volume | 110 |
Issue number | 1 |
Pages (from-to) | 39-45 |
Number of pages | 7 |
ISSN | 0167-0115 |
Publication status | Published - 31 Dec 2002 |
- Animals, Calcitonin Gene-Related Peptide, Chemotherapy, Cancer, Regional Perfusion, Duodenum, Gastric Inhibitory Polypeptide, Gastrointestinal Hormones, Glucagon, Glucagon-Like Peptide 1, Ileum, Motilin, Peptide Fragments, Postprandial Period, Protein Precursors, Sincalide, Swine
Research areas
ID: 132056393