TY - JOUR

T1 - The effect of EDTA and metal cations on the 5-bromoindoxyl acetate esterase activity in the thyroid of the guinea-pig

AU - Kirkeby, S

N1 - Keywords: Animals; Calcium; Cobalt; Edetic Acid; Enzyme Activation; Esterases; Guinea Pigs; Histocytochemistry; Magnesium; Manganese; Staining and Labeling; Thyroid Gland; Zinc

PY - 1976

Y1 - 1976

N2 - Miscellaneous metal cations and EDTA have been used as activators and inhibitors of esterase activity in the thyroid of the guinea-pig. The results indicate that the 5-bromoiondoxyl acetate esterase in the epithelial cells probably consists of two different A-esterase isoenzymes, one present in group I cells (the para-, intra-, and inter-follicular cells) and the other in group II cells (the follicular cells proper). The first isoenzyme seems to be calcium-dependent whereas the other is activated by various metal ions. Ca2+ + Mn2+ and Ca2+ + Co2+ were found to activate the esterase activity in group I cells. EDTA and Mn2+, on the other hand, activated the esterase activity in group II cells.

AB - Miscellaneous metal cations and EDTA have been used as activators and inhibitors of esterase activity in the thyroid of the guinea-pig. The results indicate that the 5-bromoiondoxyl acetate esterase in the epithelial cells probably consists of two different A-esterase isoenzymes, one present in group I cells (the para-, intra-, and inter-follicular cells) and the other in group II cells (the follicular cells proper). The first isoenzyme seems to be calcium-dependent whereas the other is activated by various metal ions. Ca2+ + Mn2+ and Ca2+ + Co2+ were found to activate the esterase activity in group I cells. EDTA and Mn2+, on the other hand, activated the esterase activity in group II cells.

M3 - Journal article

C2 - 61192

VL - 8

SP - 463

EP - 770

JO - The Histochemical Journal

JF - The Histochemical Journal

SN - 0018-2214

IS - 5

ER -