T-cell receptor excisional circles, telomere length, proliferation and apoptosis in peripheral blood mononuclear cells of human immunodeficiency virus-infected individuals after 18 months of treatment induced viral suppression
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T-cell receptor excisional circles, telomere length, proliferation and apoptosis in peripheral blood mononuclear cells of human immunodeficiency virus-infected individuals after 18 months of treatment induced viral suppression. / Aladdin, H; Katzenstein, T; Dreves, A.-M.; Ryder, L; Gerstoft, J; Skinhøj, P; Pedersen, Bente Klarlund; Ullum, H.
In: Scandinavian Journal of Immunology, Vol. 57, No. 5, 05.2003, p. 485-92.Research output: Contribution to journal › Journal article › Research › peer-review
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T1 - T-cell receptor excisional circles, telomere length, proliferation and apoptosis in peripheral blood mononuclear cells of human immunodeficiency virus-infected individuals after 18 months of treatment induced viral suppression
AU - Aladdin, H
AU - Katzenstein, T
AU - Dreves, A.-M.
AU - Ryder, L
AU - Gerstoft, J
AU - Skinhøj, P
AU - Pedersen, Bente Klarlund
AU - Ullum, H
PY - 2003/5
Y1 - 2003/5
N2 - This study evaluated the effect of highly active antiretroviral therapy (HAART)-induced viral suppression on T-cell receptor excisional circles (TRECs), telomere length, proliferative responses and spontaneous as well as phytohaemagglutinin (PHA)-stimulated lymphocyte apoptosis in 27 human immunodeficiency virus (HIV)-infected individuals followed for 18 months during HAART. Our results show that HAART significantly increased the level of TRECs in CD4+ cells (P = 0.003) after 18 months of almost continuously suppressed HIV-RNA levels. Lymphocyte proliferative responses and apoptosis levels in patients were significantly lower and significantly higher, respectively, compared with healthy controls. The proliferative response and apoptosis levels did not change during follow up. Changes in telomere length were observed in CD4+ and in CD8+ T cells. The study demonstrated that HAART induces normal TREC levels in the CD4+ T-cell pool. However, the other perturbed functions in T cells indicate that immune reconstitution is incomplete and may need longer viral suppression.
AB - This study evaluated the effect of highly active antiretroviral therapy (HAART)-induced viral suppression on T-cell receptor excisional circles (TRECs), telomere length, proliferative responses and spontaneous as well as phytohaemagglutinin (PHA)-stimulated lymphocyte apoptosis in 27 human immunodeficiency virus (HIV)-infected individuals followed for 18 months during HAART. Our results show that HAART significantly increased the level of TRECs in CD4+ cells (P = 0.003) after 18 months of almost continuously suppressed HIV-RNA levels. Lymphocyte proliferative responses and apoptosis levels in patients were significantly lower and significantly higher, respectively, compared with healthy controls. The proliferative response and apoptosis levels did not change during follow up. Changes in telomere length were observed in CD4+ and in CD8+ T cells. The study demonstrated that HAART induces normal TREC levels in the CD4+ T-cell pool. However, the other perturbed functions in T cells indicate that immune reconstitution is incomplete and may need longer viral suppression.
KW - Adult
KW - Aged
KW - Anti-HIV Agents
KW - Antiretroviral Therapy, Highly Active
KW - Apoptosis
KW - CD4 Lymphocyte Count
KW - CD4-Positive T-Lymphocytes
KW - CD8-Positive T-Lymphocytes
KW - DNA
KW - Female
KW - Follow-Up Studies
KW - Gene Rearrangement, alpha-Chain T-Cell Antigen Receptor
KW - Gene Rearrangement, beta-Chain T-Cell Antigen Receptor
KW - HIV Infections
KW - Humans
KW - Lymphocyte Activation
KW - Male
KW - Middle Aged
KW - Plasmids
KW - Receptors, Antigen, T-Cell, alpha-beta
KW - Telomere
KW - Viral Load
KW - Journal Article
M3 - Journal article
C2 - 12753506
VL - 57
SP - 485
EP - 492
JO - Scandinavian Journal of Immunology, Supplement
JF - Scandinavian Journal of Immunology, Supplement
SN - 0301-6323
IS - 5
ER -
ID: 180571386