T cell activation. II. Activation of human T lymphoma cells by cross-linking of their MHC class I antigens
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T cell activation. II. Activation of human T lymphoma cells by cross-linking of their MHC class I antigens. / Dissing, S; Geisler, C; Rubin, B; Plesner, T; Claesson, M H.
In: Cellular Immunology, Vol. 126, No. 1, 1990, p. 196-210.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - T cell activation. II. Activation of human T lymphoma cells by cross-linking of their MHC class I antigens
AU - Dissing, S
AU - Geisler, C
AU - Rubin, B
AU - Plesner, T
AU - Claesson, M H
N1 - Keywords: Antigens, CD3; Antigens, CD8; Antigens, Differentiation, T-Lymphocyte; Calcium; Histocompatibility Antigens Class I; Humans; Interleukin-2; Lymphocyte Activation; Receptors, Antigen, T-Cell; Receptors, Interleukin-2; Signal Transduction; T-Lymphocytes
PY - 1990
Y1 - 1990
N2 - The present work demonstrates that antibody-induced cross-linking of MHC class I antigens on Jurkat T lymphoma cells leads to a rise in intracellular calcium (Cai2+) and, in the presence of phorbol ester (PMA), to IL-2 production and IL-2 receptor expression. The rise in Cai2+ exhibited a profile very different from that obtained after anti-CD3 antibody-induced activation suggesting that activation signals are transduced differently after binding of anti-CD3 antibody and class I cross-linking, respectively. However, when Cai2+ was examined in individual Jurkat cells by means of a digital image processing system no differences were observed after cross-linking with anti-CD3 and anti-MHC class I antibodies, respectively. Two CD3-negative mutant lymphoma lines were nearly totally refractory to class I cross-linking. Taken together our results may indicate the existence of a functional linkage between the T cell receptor complex and MHC class I molecules.
AB - The present work demonstrates that antibody-induced cross-linking of MHC class I antigens on Jurkat T lymphoma cells leads to a rise in intracellular calcium (Cai2+) and, in the presence of phorbol ester (PMA), to IL-2 production and IL-2 receptor expression. The rise in Cai2+ exhibited a profile very different from that obtained after anti-CD3 antibody-induced activation suggesting that activation signals are transduced differently after binding of anti-CD3 antibody and class I cross-linking, respectively. However, when Cai2+ was examined in individual Jurkat cells by means of a digital image processing system no differences were observed after cross-linking with anti-CD3 and anti-MHC class I antibodies, respectively. Two CD3-negative mutant lymphoma lines were nearly totally refractory to class I cross-linking. Taken together our results may indicate the existence of a functional linkage between the T cell receptor complex and MHC class I molecules.
M3 - Journal article
C2 - 2137376
VL - 126
SP - 196
EP - 210
JO - Cellular Immunology
JF - Cellular Immunology
SN - 0008-8749
IS - 1
ER -
ID: 8546392