Subclassification of cells in S phase in a partially synchronized cell system. Methodological problems concerning pulse labelling of epithelial cells in the hamster cheek pouch

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Subclassification of cells in S phase in a partially synchronized cell system. Methodological problems concerning pulse labelling of epithelial cells in the hamster cheek pouch. / Møller, U.; Keiding, N.; Engel, F.

In: Cell and Tissue Kinetics, Vol. 15, No. 2, 03.1982, p. 157-168.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Møller, U, Keiding, N & Engel, F 1982, 'Subclassification of cells in S phase in a partially synchronized cell system. Methodological problems concerning pulse labelling of epithelial cells in the hamster cheek pouch', Cell and Tissue Kinetics, vol. 15, no. 2, pp. 157-168. https://doi.org/10.1111/j.1365-2184.1982.tb01034.x

APA

Møller, U., Keiding, N., & Engel, F. (1982). Subclassification of cells in S phase in a partially synchronized cell system. Methodological problems concerning pulse labelling of epithelial cells in the hamster cheek pouch. Cell and Tissue Kinetics, 15(2), 157-168. https://doi.org/10.1111/j.1365-2184.1982.tb01034.x

Vancouver

Møller U, Keiding N, Engel F. Subclassification of cells in S phase in a partially synchronized cell system. Methodological problems concerning pulse labelling of epithelial cells in the hamster cheek pouch. Cell and Tissue Kinetics. 1982 Mar;15(2):157-168. https://doi.org/10.1111/j.1365-2184.1982.tb01034.x

Author

Møller, U. ; Keiding, N. ; Engel, F. / Subclassification of cells in S phase in a partially synchronized cell system. Methodological problems concerning pulse labelling of epithelial cells in the hamster cheek pouch. In: Cell and Tissue Kinetics. 1982 ; Vol. 15, No. 2. pp. 157-168.

Bibtex

@article{f8e50ea31a02468b9d393052aff60fab,
title = "Subclassification of cells in S phase in a partially synchronized cell system.: Methodological problems concerning pulse labelling of epithelial cells in the hamster cheek pouch",
abstract = "A circadian dependent delay in the incorporation of [ 3H]TdR into DNA, presumably due to variations in the intracellular pool of [ 3H]TdR derivatives, was found. It seems reasonable to relate this effect to a circadianally varying age distribution of cells in S phase. At any given time the S phase cells showed large variations in DNA synthesis rate, but it was still possible to identify a mean diurnal variation in the DNA synthesis rate. Differences in the ability of S phase cells to incorporate [ 3H]TdR are also discussed in relation to flow cytometrical measurements, and this contributes to the understanding of the commonly observed phenomenon that flow cytometry estimates of S-fractions are higher than those obtained with autoradiography.",
author = "U. M{\o}ller and N. Keiding and F. Engel",
year = "1982",
month = mar,
doi = "10.1111/j.1365-2184.1982.tb01034.x",
language = "English",
volume = "15",
pages = "157--168",
journal = "Cell and Tissue Kinetics",
issn = "0008-8730",
publisher = "Wiley-Blackwell",
number = "2",

}

RIS

TY - JOUR

T1 - Subclassification of cells in S phase in a partially synchronized cell system.

T2 - Methodological problems concerning pulse labelling of epithelial cells in the hamster cheek pouch

AU - Møller, U.

AU - Keiding, N.

AU - Engel, F.

PY - 1982/3

Y1 - 1982/3

N2 - A circadian dependent delay in the incorporation of [ 3H]TdR into DNA, presumably due to variations in the intracellular pool of [ 3H]TdR derivatives, was found. It seems reasonable to relate this effect to a circadianally varying age distribution of cells in S phase. At any given time the S phase cells showed large variations in DNA synthesis rate, but it was still possible to identify a mean diurnal variation in the DNA synthesis rate. Differences in the ability of S phase cells to incorporate [ 3H]TdR are also discussed in relation to flow cytometrical measurements, and this contributes to the understanding of the commonly observed phenomenon that flow cytometry estimates of S-fractions are higher than those obtained with autoradiography.

AB - A circadian dependent delay in the incorporation of [ 3H]TdR into DNA, presumably due to variations in the intracellular pool of [ 3H]TdR derivatives, was found. It seems reasonable to relate this effect to a circadianally varying age distribution of cells in S phase. At any given time the S phase cells showed large variations in DNA synthesis rate, but it was still possible to identify a mean diurnal variation in the DNA synthesis rate. Differences in the ability of S phase cells to incorporate [ 3H]TdR are also discussed in relation to flow cytometrical measurements, and this contributes to the understanding of the commonly observed phenomenon that flow cytometry estimates of S-fractions are higher than those obtained with autoradiography.

UR - http://www.scopus.com/inward/record.url?scp=0020038983&partnerID=8YFLogxK

U2 - 10.1111/j.1365-2184.1982.tb01034.x

DO - 10.1111/j.1365-2184.1982.tb01034.x

M3 - Journal article

C2 - 7066958

VL - 15

SP - 157

EP - 168

JO - Cell and Tissue Kinetics

JF - Cell and Tissue Kinetics

SN - 0008-8730

IS - 2

ER -

ID: 203011424