Strontium ion reinforced bioceramic scaffold for load bearing bone regeneration
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Strontium ion reinforced bioceramic scaffold for load bearing bone regeneration. / Prabha, R. D.; Ding, Ming; Bollen, P.; Ditzel, N.; Varma, H. K.; Nair, P. D.; Kassem, M.
In: Materials Science and Engineering C: Materials for Biological Applications, Vol. 109, 110427, 04.2020.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Strontium ion reinforced bioceramic scaffold for load bearing bone regeneration
AU - Prabha, R. D.
AU - Ding, Ming
AU - Bollen, P.
AU - Ditzel, N.
AU - Varma, H. K.
AU - Nair, P. D.
AU - Kassem, M.
PY - 2020/4
Y1 - 2020/4
N2 - Bone defects in load bearing areas require bone reconstruction with strong biomaterial having mechanical characteristics like cortical bone. Bioceramics are biomaterials that support bone formation as well as provide adequate mechanical properties. A strontium substitution of the bioceramic is expected to further increase its bioactivity by enhancing osteogenesis and protect the bone from osteoclastic resorption. The study involves development, characterization and in vivo testing of a newly developed strontium substituted hydroxyapatite based bioceramic scaffold (SrHAB) with sufficient biomechanical properties. Optimal concentration of strontium ion required for enhanced osteogenic differentiation was identified by comparing three compositions of SrHAB scaffold; namely Sri10HAB, Sr30HAB and Sr50 HAB for their Alkaline phosphatase activity in vitro. The selected Sr10HAB scaffold demonstrated in vivo bone formation with osteogenic differentiation of stromal derived mesenchymal stem cells (MSC) from human and ovine sources in ectopic and ovine models. Thus, Sr10HAB scaffold has a potential for application in load bearing bone requirements of orthopaedics and dentistry.
AB - Bone defects in load bearing areas require bone reconstruction with strong biomaterial having mechanical characteristics like cortical bone. Bioceramics are biomaterials that support bone formation as well as provide adequate mechanical properties. A strontium substitution of the bioceramic is expected to further increase its bioactivity by enhancing osteogenesis and protect the bone from osteoclastic resorption. The study involves development, characterization and in vivo testing of a newly developed strontium substituted hydroxyapatite based bioceramic scaffold (SrHAB) with sufficient biomechanical properties. Optimal concentration of strontium ion required for enhanced osteogenic differentiation was identified by comparing three compositions of SrHAB scaffold; namely Sri10HAB, Sr30HAB and Sr50 HAB for their Alkaline phosphatase activity in vitro. The selected Sr10HAB scaffold demonstrated in vivo bone formation with osteogenic differentiation of stromal derived mesenchymal stem cells (MSC) from human and ovine sources in ectopic and ovine models. Thus, Sr10HAB scaffold has a potential for application in load bearing bone requirements of orthopaedics and dentistry.
KW - Load bearing
KW - In vivo
KW - Bio ceramic
KW - Bone
KW - Strontium
KW - BIOACTIVE GLASSES
KW - DIFFERENTIATION
KW - OSTEOPONTIN
KW - GROWTH
KW - PHOSPHORYLATION
KW - RECONSTRUCTION
KW - ANGIOGENESIS
KW - RESORPTION
KW - INCREASES
KW - RANELATE
U2 - 10.1016/j.msec.2019.110427
DO - 10.1016/j.msec.2019.110427
M3 - Journal article
C2 - 32228983
VL - 109
JO - Materials Science and Engineering C: Materials for Biological Applications
JF - Materials Science and Engineering C: Materials for Biological Applications
SN - 1873-0191
M1 - 110427
ER -
ID: 257242352