Stimulation of the N-methyl-D-aspartate receptor has a trophic effect on differentiating cerebellar granule cells.
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Stimulation of the N-methyl-D-aspartate receptor has a trophic effect on differentiating cerebellar granule cells. / Balázs, R; Hack, N; Jørgensen, Ole Steen.
In: Neuroscience Letters, Vol. 87, No. 1-2, 1988, p. 80-6.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Stimulation of the N-methyl-D-aspartate receptor has a trophic effect on differentiating cerebellar granule cells.
AU - Balázs, R
AU - Hack, N
AU - Jørgensen, Ole Steen
N1 - Keywords: Animals; Antigens, Surface; Aspartic Acid; Cell Adhesion Molecules; Cell Count; Cell Differentiation; Cell Survival; Cells, Cultured; Cerebellum; N-Methylaspartate; Rats; Receptors, N-Methyl-D-Aspartate; Receptors, Neurotransmitter; Tetanus Toxin
PY - 1988
Y1 - 1988
N2 - N-methyl-D-aspartate (NMDA) supplementation of cerebellar cultures enriched in granule neurones (about 90%) prevented the extensive cell loss which occurs when cultivation takes place, in serum containing media, in the presence of 'low' K+ (5-15 mM). Estimation of tetanus toxin receptors and N-CAM contents indicated that NMDA rescued primarily nerve cells. The influence of NMDA in promoting cell survival was blocked by the receptor antagonist, 2-amino-5-phosphonovalerate. The effect depended both on the concentration of NMDA and on the degree of depolarization of cells, the affinity in the presence of 15 mM K+ being similar to that of NMDA receptor binding. The results attest a new role for excitatory amino acid transmitters by showing that they can exert a stage-dependent trophic action on developing nerve cells.
AB - N-methyl-D-aspartate (NMDA) supplementation of cerebellar cultures enriched in granule neurones (about 90%) prevented the extensive cell loss which occurs when cultivation takes place, in serum containing media, in the presence of 'low' K+ (5-15 mM). Estimation of tetanus toxin receptors and N-CAM contents indicated that NMDA rescued primarily nerve cells. The influence of NMDA in promoting cell survival was blocked by the receptor antagonist, 2-amino-5-phosphonovalerate. The effect depended both on the concentration of NMDA and on the degree of depolarization of cells, the affinity in the presence of 15 mM K+ being similar to that of NMDA receptor binding. The results attest a new role for excitatory amino acid transmitters by showing that they can exert a stage-dependent trophic action on developing nerve cells.
M3 - Journal article
C2 - 2837687
VL - 87
SP - 80
EP - 86
JO - Neuroscience letters. Supplement
JF - Neuroscience letters. Supplement
SN - 0167-6253
IS - 1-2
ER -
ID: 5941375