Stella modulates transcriptional and endogenous retrovirus programs during maternal-to-zygotic transition
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Stella modulates transcriptional and endogenous retrovirus programs during maternal-to-zygotic transition. / Huang, Yun; Kim, Jong Kyoung; Do, Dang Vinh; Lee, Caroline; Penfold, Christopher A.; Zylicz, Jan J.; Marioni, John C.; Hackett, Jamie A.; Surani, M. Azim.
In: eLife, Vol. 6, e22345, 21.03.2017.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Stella modulates transcriptional and endogenous retrovirus programs during maternal-to-zygotic transition
AU - Huang, Yun
AU - Kim, Jong Kyoung
AU - Do, Dang Vinh
AU - Lee, Caroline
AU - Penfold, Christopher A.
AU - Zylicz, Jan J.
AU - Marioni, John C.
AU - Hackett, Jamie A.
AU - Surani, M. Azim
N1 - Funding Information: We thank Richard Butler for his support on the confocal imaging analysis, Charles Bradshaw for bioinformatic support, Todd S Macfarlan and Samuel L Pfaff for the 2C::tdTomato ESCs. We also thank members of the Surani lab for their critical input and helpful discussions on this project. The work was funded by a studentship to YH from the James Baird Fund, University of Cambridge, by the DGIST Start-up Fund of the Ministry of Science, ICT and Future Planning to JKK, by a core grant from EMBL and CRUK to JCM, by a Wellcome Trust Senior Investigator Award to MAS, and by a core grant from the Wellcome Trust and Cancer Research UK to the Gurdon Institute. Publisher Copyright: © Huang et al.
PY - 2017/3/21
Y1 - 2017/3/21
N2 - The maternal-to-zygotic transition (MZT) marks the period when the embryonic genome is activated and acquires control of development. Maternally inherited factors play a key role in this critical developmental process, which occurs at the 2-cell stage in mice. We investigated the function of the maternally inherited factor Stella (encoded by Dppa3) using single-cell/embryo approaches. We show that loss of maternal Stella results in widespread transcriptional misregulation and a partial failure of MZT. Strikingly, activation of endogenous retroviruses (ERVs) is significantly impaired in Stella maternal/zygotic knockout embryos, which in turn leads to a failure to upregulate chimeric transcripts. Amongst ERVs, MuERV-L activation is particularly affected by the absence of Stella, and direct in vivo knockdown of MuERV-L impacts the developmental potential of the embryo. We propose that Stella is involved in ensuring activation of ERVs, which themselves play a potentially key role during early development, either directly or through influencing embryonic gene expression.
AB - The maternal-to-zygotic transition (MZT) marks the period when the embryonic genome is activated and acquires control of development. Maternally inherited factors play a key role in this critical developmental process, which occurs at the 2-cell stage in mice. We investigated the function of the maternally inherited factor Stella (encoded by Dppa3) using single-cell/embryo approaches. We show that loss of maternal Stella results in widespread transcriptional misregulation and a partial failure of MZT. Strikingly, activation of endogenous retroviruses (ERVs) is significantly impaired in Stella maternal/zygotic knockout embryos, which in turn leads to a failure to upregulate chimeric transcripts. Amongst ERVs, MuERV-L activation is particularly affected by the absence of Stella, and direct in vivo knockdown of MuERV-L impacts the developmental potential of the embryo. We propose that Stella is involved in ensuring activation of ERVs, which themselves play a potentially key role during early development, either directly or through influencing embryonic gene expression.
UR - http://www.scopus.com/inward/record.url?scp=85018265565&partnerID=8YFLogxK
U2 - 10.7554/eLife.22345
DO - 10.7554/eLife.22345
M3 - Journal article
C2 - 28323615
AN - SCOPUS:85018265565
VL - 6
JO - eLife
JF - eLife
SN - 2050-084X
M1 - e22345
ER -
ID: 391637999