Standard methods for fungal brood disease research
Research output: Contribution to journal › Review › Research › peer-review
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Standard methods for fungal brood disease research. / Jensen, Annette Bruun; Aronstein, Kathrine; Manuel Flores, Jose; Vojvodic, Svjetlana; Palacio, Maria Alejandra; Spivak, Marla.
In: Journal of Apicultural Research & Bee World, Vol. 52, No. 1, 2013.Research output: Contribution to journal › Review › Research › peer-review
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TY - JOUR
T1 - Standard methods for fungal brood disease research
AU - Jensen, Annette Bruun
AU - Aronstein, Kathrine
AU - Manuel Flores, Jose
AU - Vojvodic, Svjetlana
AU - Palacio, Maria Alejandra
AU - Spivak, Marla
PY - 2013
Y1 - 2013
N2 - Chalkbrood and stonebrood are two fungal diseases associated with honey bee brood. Chalkbrood, caused by Ascosphaera apis, is a common and widespread disease that can result in severe reduction of emerging worker bees and thus overall colony productivity. Stonebrood is caused by Aspergillus spp. that are rarely observed, so the impact on colony health is not very well understood. A major concern with the presence of Aspergillus in honey bees is the production of airborne conidia, which can lead to allergic bronchopulmonary aspergillosis, pulmonary aspergilloma, or even invasive aspergillosis in lung tissues upon inhalation by humans. In the current chapter we describe the honey bee disease symptoms of these fungal pathogens. In addition, we provide research methodologies and protocols for isolating and culturing, in vivo and in vitro assays that are commonly used to study these host pathogen interactions. We give guidelines on the preferred methods used in current research and the application of molecular techniques. We have added photographs, drawings and illustrations to assist bee-extension personnel and bee scientists in the control of these two diseases.
AB - Chalkbrood and stonebrood are two fungal diseases associated with honey bee brood. Chalkbrood, caused by Ascosphaera apis, is a common and widespread disease that can result in severe reduction of emerging worker bees and thus overall colony productivity. Stonebrood is caused by Aspergillus spp. that are rarely observed, so the impact on colony health is not very well understood. A major concern with the presence of Aspergillus in honey bees is the production of airborne conidia, which can lead to allergic bronchopulmonary aspergillosis, pulmonary aspergilloma, or even invasive aspergillosis in lung tissues upon inhalation by humans. In the current chapter we describe the honey bee disease symptoms of these fungal pathogens. In addition, we provide research methodologies and protocols for isolating and culturing, in vivo and in vitro assays that are commonly used to study these host pathogen interactions. We give guidelines on the preferred methods used in current research and the application of molecular techniques. We have added photographs, drawings and illustrations to assist bee-extension personnel and bee scientists in the control of these two diseases.
KW - Ascosphaera
KW - Ascosphaerales
KW - Aspergillus
KW - chalkbrood
KW - diagnostics
KW - methods
KW - recommendations
KW - stonebrood
KW - COLOSS
KW - BEEBOOK
KW - honey bee
U2 - 10.3896/IBRA.1.52.1.13
DO - 10.3896/IBRA.1.52.1.13
M3 - Review
C2 - 24198438
VL - 52
JO - Journal of Apicultural Research & Bee World
JF - Journal of Apicultural Research & Bee World
SN - 1751-2891
IS - 1
ER -
ID: 117864800