Stability and replication control of Escherichia coli minichromosomes

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Stability and replication control of Escherichia coli minichromosomes. / Løbner-Olesen, A; Atlung, T; Rasmussen, K V.

In: Journal of Bacteriology, Vol. 169, No. 6, 06.1987, p. 2835-42.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Løbner-Olesen, A, Atlung, T & Rasmussen, KV 1987, 'Stability and replication control of Escherichia coli minichromosomes', Journal of Bacteriology, vol. 169, no. 6, pp. 2835-42.

APA

Løbner-Olesen, A., Atlung, T., & Rasmussen, K. V. (1987). Stability and replication control of Escherichia coli minichromosomes. Journal of Bacteriology, 169(6), 2835-42.

Vancouver

Løbner-Olesen A, Atlung T, Rasmussen KV. Stability and replication control of Escherichia coli minichromosomes. Journal of Bacteriology. 1987 Jun;169(6):2835-42.

Author

Løbner-Olesen, A ; Atlung, T ; Rasmussen, K V. / Stability and replication control of Escherichia coli minichromosomes. In: Journal of Bacteriology. 1987 ; Vol. 169, No. 6. pp. 2835-42.

Bibtex

@article{aab2ac2719e64df98ebe786afbb2bd38,
title = "Stability and replication control of Escherichia coli minichromosomes",
abstract = "A stabilized minichromosome--a plasmid replicating from the chromosomal origin oriC--was constructed by cloning the sopA,B,C, genes from plasmid F. This minichromosome had a loss frequency of less than 10(-3), while that of the nonstabilized parental plasmid was 2 X 10(-2) to 4 X 10(-2). Both minichromosomes had the same average copy number per chromosomal origin, and the copy numbers were constant over an eightfold range of growth rates. Different mutations in the mioC gene and promoter, from which transcription enters oriC, were constructed, and their effects on minichromosome copy number and stability were tested. The results indicated that normal replication control at oriC was independent of the MioC protein and most of the sequences between the promoter and oriC, but required both transcription from the mioC promoter and probably also the presence of the DnaA box (DnaA protein-binding site) just upstream of the mioC promoter. Transcription from the mioC promoter was shown to be efficiently repressed in vivo after overproduction of DnaA protein and to be derepressed at the nonpermissive temperature in six different dnaA(Ts) mutants.",
keywords = "Bacterial Proteins/physiology, Chromosomes, Bacterial/physiology, DNA Replication, Escherichia coli/genetics, Gene Expression Regulation, Genes, Bacterial, Plasmids, Promoter Regions, Genetic, Transcription, Genetic",
author = "A L{\o}bner-Olesen and T Atlung and Rasmussen, {K V}",
year = "1987",
month = jun,
language = "English",
volume = "169",
pages = "2835--42",
journal = "Journal of Bacteriology",
issn = "0021-9193",
publisher = "American Society for Microbiology",
number = "6",

}

RIS

TY - JOUR

T1 - Stability and replication control of Escherichia coli minichromosomes

AU - Løbner-Olesen, A

AU - Atlung, T

AU - Rasmussen, K V

PY - 1987/6

Y1 - 1987/6

N2 - A stabilized minichromosome--a plasmid replicating from the chromosomal origin oriC--was constructed by cloning the sopA,B,C, genes from plasmid F. This minichromosome had a loss frequency of less than 10(-3), while that of the nonstabilized parental plasmid was 2 X 10(-2) to 4 X 10(-2). Both minichromosomes had the same average copy number per chromosomal origin, and the copy numbers were constant over an eightfold range of growth rates. Different mutations in the mioC gene and promoter, from which transcription enters oriC, were constructed, and their effects on minichromosome copy number and stability were tested. The results indicated that normal replication control at oriC was independent of the MioC protein and most of the sequences between the promoter and oriC, but required both transcription from the mioC promoter and probably also the presence of the DnaA box (DnaA protein-binding site) just upstream of the mioC promoter. Transcription from the mioC promoter was shown to be efficiently repressed in vivo after overproduction of DnaA protein and to be derepressed at the nonpermissive temperature in six different dnaA(Ts) mutants.

AB - A stabilized minichromosome--a plasmid replicating from the chromosomal origin oriC--was constructed by cloning the sopA,B,C, genes from plasmid F. This minichromosome had a loss frequency of less than 10(-3), while that of the nonstabilized parental plasmid was 2 X 10(-2) to 4 X 10(-2). Both minichromosomes had the same average copy number per chromosomal origin, and the copy numbers were constant over an eightfold range of growth rates. Different mutations in the mioC gene and promoter, from which transcription enters oriC, were constructed, and their effects on minichromosome copy number and stability were tested. The results indicated that normal replication control at oriC was independent of the MioC protein and most of the sequences between the promoter and oriC, but required both transcription from the mioC promoter and probably also the presence of the DnaA box (DnaA protein-binding site) just upstream of the mioC promoter. Transcription from the mioC promoter was shown to be efficiently repressed in vivo after overproduction of DnaA protein and to be derepressed at the nonpermissive temperature in six different dnaA(Ts) mutants.

KW - Bacterial Proteins/physiology

KW - Chromosomes, Bacterial/physiology

KW - DNA Replication

KW - Escherichia coli/genetics

KW - Gene Expression Regulation

KW - Genes, Bacterial

KW - Plasmids

KW - Promoter Regions, Genetic

KW - Transcription, Genetic

M3 - Journal article

C2 - 3294807

VL - 169

SP - 2835

EP - 2842

JO - Journal of Bacteriology

JF - Journal of Bacteriology

SN - 0021-9193

IS - 6

ER -

ID: 200973510