Spreading of Sir3 protein in cells with severe histone H3 hypoacetylation

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Spreading of Sir3 protein in cells with severe histone H3 hypoacetylation. / Kristjuhan, Arnold; Wittschieben, Birgitte; Walker, Jane; Roberts, Douglas; Cairns, Bradley R.; Svejstrup, Jesper Q.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 100, No. 13, 24.06.2003, p. 7551-7556.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Kristjuhan, A, Wittschieben, B, Walker, J, Roberts, D, Cairns, BR & Svejstrup, JQ 2003, 'Spreading of Sir3 protein in cells with severe histone H3 hypoacetylation', Proceedings of the National Academy of Sciences of the United States of America, vol. 100, no. 13, pp. 7551-7556. https://doi.org/10.1073/pnas.1332299100

APA

Kristjuhan, A., Wittschieben, B., Walker, J., Roberts, D., Cairns, B. R., & Svejstrup, J. Q. (2003). Spreading of Sir3 protein in cells with severe histone H3 hypoacetylation. Proceedings of the National Academy of Sciences of the United States of America, 100(13), 7551-7556. https://doi.org/10.1073/pnas.1332299100

Vancouver

Kristjuhan A, Wittschieben B, Walker J, Roberts D, Cairns BR, Svejstrup JQ. Spreading of Sir3 protein in cells with severe histone H3 hypoacetylation. Proceedings of the National Academy of Sciences of the United States of America. 2003 Jun 24;100(13):7551-7556. https://doi.org/10.1073/pnas.1332299100

Author

Kristjuhan, Arnold ; Wittschieben, Birgitte ; Walker, Jane ; Roberts, Douglas ; Cairns, Bradley R. ; Svejstrup, Jesper Q. / Spreading of Sir3 protein in cells with severe histone H3 hypoacetylation. In: Proceedings of the National Academy of Sciences of the United States of America. 2003 ; Vol. 100, No. 13. pp. 7551-7556.

Bibtex

@article{cb3a52dca10d475bb797b1ae0c03d2a9,
title = "Spreading of Sir3 protein in cells with severe histone H3 hypoacetylation",
abstract = "Heterochromatin formation in yeast involves deacetylation of histones, but the precise relationship between acetylation and the association of proteins such as Sir3, Sir4, and the histone deacetylase Sir2 with chromatin is still unclear. Here we show that Sir3 protein spreads to subtelomeric DNA in cells lacking the transcription-related histone acetyltransferases GCN5 and ELP3. Spreading correlates with hypoacetylation of lysines in the histone H3 tail and results in deacetylation of lysine 16 in histone H4. De-repression of genes situated very close to the ends of the chromosomes in gcn5 elp3 suggests that Sir3 spreads into subtelomeric DNA from the tip of the telomere. Interestingly, growth defects caused by gcn5 elp3 mutation can be suppressed by SIR deletion, suggesting that Sir proteins become detrimental for growth when chromatin is severely hypoacetylated.",
author = "Arnold Kristjuhan and Birgitte Wittschieben and Jane Walker and Douglas Roberts and Cairns, {Bradley R.} and Svejstrup, {Jesper Q.}",
year = "2003",
month = jun,
day = "24",
doi = "10.1073/pnas.1332299100",
language = "English",
volume = "100",
pages = "7551--7556",
journal = "Proceedings of the National Academy of Sciences of the United States of America",
issn = "0027-8424",
publisher = "The National Academy of Sciences of the United States of America",
number = "13",

}

RIS

TY - JOUR

T1 - Spreading of Sir3 protein in cells with severe histone H3 hypoacetylation

AU - Kristjuhan, Arnold

AU - Wittschieben, Birgitte

AU - Walker, Jane

AU - Roberts, Douglas

AU - Cairns, Bradley R.

AU - Svejstrup, Jesper Q.

PY - 2003/6/24

Y1 - 2003/6/24

N2 - Heterochromatin formation in yeast involves deacetylation of histones, but the precise relationship between acetylation and the association of proteins such as Sir3, Sir4, and the histone deacetylase Sir2 with chromatin is still unclear. Here we show that Sir3 protein spreads to subtelomeric DNA in cells lacking the transcription-related histone acetyltransferases GCN5 and ELP3. Spreading correlates with hypoacetylation of lysines in the histone H3 tail and results in deacetylation of lysine 16 in histone H4. De-repression of genes situated very close to the ends of the chromosomes in gcn5 elp3 suggests that Sir3 spreads into subtelomeric DNA from the tip of the telomere. Interestingly, growth defects caused by gcn5 elp3 mutation can be suppressed by SIR deletion, suggesting that Sir proteins become detrimental for growth when chromatin is severely hypoacetylated.

AB - Heterochromatin formation in yeast involves deacetylation of histones, but the precise relationship between acetylation and the association of proteins such as Sir3, Sir4, and the histone deacetylase Sir2 with chromatin is still unclear. Here we show that Sir3 protein spreads to subtelomeric DNA in cells lacking the transcription-related histone acetyltransferases GCN5 and ELP3. Spreading correlates with hypoacetylation of lysines in the histone H3 tail and results in deacetylation of lysine 16 in histone H4. De-repression of genes situated very close to the ends of the chromosomes in gcn5 elp3 suggests that Sir3 spreads into subtelomeric DNA from the tip of the telomere. Interestingly, growth defects caused by gcn5 elp3 mutation can be suppressed by SIR deletion, suggesting that Sir proteins become detrimental for growth when chromatin is severely hypoacetylated.

U2 - 10.1073/pnas.1332299100

DO - 10.1073/pnas.1332299100

M3 - Journal article

C2 - 12796514

AN - SCOPUS:0038610948

VL - 100

SP - 7551

EP - 7556

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

SN - 0027-8424

IS - 13

ER -

ID: 331041530