Spatial transcriptome unveils a discontinuous inflammatory pattern in proficient mismatch repair colorectal adenocarcinoma
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Spatial transcriptome unveils a discontinuous inflammatory pattern in proficient mismatch repair colorectal adenocarcinoma. / Zhang, Rongxin; Feng, Yu; Ma, Wenjuan; Guo, Yanying; Luo, Mei; Li, Young; Zang, Yupeng; Dong, Xuan; Lu, Shixun; Guo, Qiang; Xu, Qumiao; Chen, Huanyi; Li, Yijian; Liu, Longqi; Chen, Ao; Chen, Gong; Xu, Xun.
In: Fundamental Research, Vol. 3, No. 4, 2023.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Spatial transcriptome unveils a discontinuous inflammatory pattern in proficient mismatch repair colorectal adenocarcinoma
AU - Zhang, Rongxin
AU - Feng, Yu
AU - Ma, Wenjuan
AU - Guo, Yanying
AU - Luo, Mei
AU - Li, Young
AU - Zang, Yupeng
AU - Dong, Xuan
AU - Lu, Shixun
AU - Guo, Qiang
AU - Xu, Qumiao
AU - Chen, Huanyi
AU - Li, Yijian
AU - Liu, Longqi
AU - Chen, Ao
AU - Chen, Gong
AU - Xu, Xun
N1 - Publisher Copyright: © 2022
PY - 2023
Y1 - 2023
N2 - The preexistence of immune cells in the tumor microenvironment substantiates the efficacy of immunotherapy in cancer patients. Although the complex intratumoral immune heterogeneity has been extensively studied in single cell resolution, hi-res spatial investigations are limited. In this study, we performed a spatial transcriptome analysis of 4 colorectal adenocarcinoma specimens and 2 paired distant normal specimens to identify the molecular pattern involved in a discontinuous inflammatory response in pathologically annotated cancer regions. Based on the location of spatially varied gene expression, we unmasked the spatially-varied immune ecosystem and identified the locoregional “warmed-up” immune response in predefined “cold” tumor with substantial infiltration of immune components. This “warmed-up” immune profile was found to be associated with the in-situ copy number variance and the tissue remodeling process. Further, “warmed-up” signature genes indicated improved overall survival in CRC patients obtained from TCGA database.
AB - The preexistence of immune cells in the tumor microenvironment substantiates the efficacy of immunotherapy in cancer patients. Although the complex intratumoral immune heterogeneity has been extensively studied in single cell resolution, hi-res spatial investigations are limited. In this study, we performed a spatial transcriptome analysis of 4 colorectal adenocarcinoma specimens and 2 paired distant normal specimens to identify the molecular pattern involved in a discontinuous inflammatory response in pathologically annotated cancer regions. Based on the location of spatially varied gene expression, we unmasked the spatially-varied immune ecosystem and identified the locoregional “warmed-up” immune response in predefined “cold” tumor with substantial infiltration of immune components. This “warmed-up” immune profile was found to be associated with the in-situ copy number variance and the tissue remodeling process. Further, “warmed-up” signature genes indicated improved overall survival in CRC patients obtained from TCGA database.
KW - Colorectal adenocarcinoma
KW - Inflammation
KW - Mismatch repair
KW - Spatial transcriptomics
KW - Tumor microenvironment
U2 - 10.1016/j.fmre.2022.01.036
DO - 10.1016/j.fmre.2022.01.036
M3 - Journal article
C2 - 38933545
AN - SCOPUS:85128285510
VL - 3
JO - Fundamental Research
JF - Fundamental Research
SN - 2667-3258
IS - 4
ER -
ID: 310558072