Somatic STAT3 mutations in large granular lymphocytic leukemia
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Somatic STAT3 mutations in large granular lymphocytic leukemia. / Koskela, Hanna L M; Eldfors, Samuli; Ellonen, Pekka; van Adrichem, Arjan J; Kuusanmäki, Heikki; Andersson, Emma I; Lagström, Sonja; Clemente, Michael J; Olson, Thomas; Jalkanen, Sari E; Majumder, Muntasir Mamun; Almusa, Henrikki; Edgren, Henrik; Lepistö, Maija; Mattila, Pirkko; Guinta, Kathryn; Koistinen, Pirjo; Kuittinen, Taru; Penttinen, Kati; Parsons, Alun; Knowles, Jonathan; Saarela, Janna; Wennerberg, Krister; Kallioniemi, Olli; Porkka, Kimmo; Loughran, Thomas P; Heckman, Caroline A; Maciejewski, Jaroslaw P; Mustjoki, Satu.
In: The New England Journal of Medicine, Vol. 366, No. 20, 17.05.2012, p. 1905-13.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Somatic STAT3 mutations in large granular lymphocytic leukemia
AU - Koskela, Hanna L M
AU - Eldfors, Samuli
AU - Ellonen, Pekka
AU - van Adrichem, Arjan J
AU - Kuusanmäki, Heikki
AU - Andersson, Emma I
AU - Lagström, Sonja
AU - Clemente, Michael J
AU - Olson, Thomas
AU - Jalkanen, Sari E
AU - Majumder, Muntasir Mamun
AU - Almusa, Henrikki
AU - Edgren, Henrik
AU - Lepistö, Maija
AU - Mattila, Pirkko
AU - Guinta, Kathryn
AU - Koistinen, Pirjo
AU - Kuittinen, Taru
AU - Penttinen, Kati
AU - Parsons, Alun
AU - Knowles, Jonathan
AU - Saarela, Janna
AU - Wennerberg, Krister
AU - Kallioniemi, Olli
AU - Porkka, Kimmo
AU - Loughran, Thomas P
AU - Heckman, Caroline A
AU - Maciejewski, Jaroslaw P
AU - Mustjoki, Satu
PY - 2012/5/17
Y1 - 2012/5/17
N2 - BACKGROUND: T-cell large granular lymphocytic leukemia is a rare lymphoproliferative disorder characterized by the expansion of clonal CD3+CD8+ cytotoxic T lymphocytes (CTLs) and often associated with autoimmune disorders and immune-mediated cytopenias.METHODS: We used next-generation exome sequencing to identify somatic mutations in CTLs from an index patient with large granular lymphocytic leukemia. Targeted resequencing was performed in a well-characterized cohort of 76 patients with this disorder, characterized by clonal T-cell-receptor rearrangements and increased numbers of large granular lymphocytes.RESULTS: Mutations in the signal transducer and activator of transcription 3 gene (STAT3) were found in 31 of 77 patients (40%) with large granular lymphocytic leukemia. Among these 31 patients, recurrent mutational hot spots included Y640F in 13 (17%), D661V in 7 (9%), D661Y in 7 (9%), and N647I in 3 (4%). All mutations were located in exon 21, encoding the Src homology 2 (SH2) domain, which mediates the dimerization and activation of STAT protein. The amino acid changes resulted in a more hydrophobic protein surface and were associated with phosphorylation of STAT3 and its localization in the nucleus. In vitro functional studies showed that the Y640F and D661V mutations increased the transcriptional activity of STAT3. In the affected patients, downstream target genes of the STAT3 pathway (IFNGR2, BCL2L1, and JAK2) were up-regulated. Patients with STAT3 mutations presented more often with neutropenia and rheumatoid arthritis than did patients without these mutations.CONCLUSIONS: The SH2 dimerization and activation domain of STAT3 is frequently mutated in patients with large granular lymphocytic leukemia; these findings suggest that aberrant STAT3 signaling underlies the pathogenesis of this disease. (Funded by the Academy of Finland and others.).
AB - BACKGROUND: T-cell large granular lymphocytic leukemia is a rare lymphoproliferative disorder characterized by the expansion of clonal CD3+CD8+ cytotoxic T lymphocytes (CTLs) and often associated with autoimmune disorders and immune-mediated cytopenias.METHODS: We used next-generation exome sequencing to identify somatic mutations in CTLs from an index patient with large granular lymphocytic leukemia. Targeted resequencing was performed in a well-characterized cohort of 76 patients with this disorder, characterized by clonal T-cell-receptor rearrangements and increased numbers of large granular lymphocytes.RESULTS: Mutations in the signal transducer and activator of transcription 3 gene (STAT3) were found in 31 of 77 patients (40%) with large granular lymphocytic leukemia. Among these 31 patients, recurrent mutational hot spots included Y640F in 13 (17%), D661V in 7 (9%), D661Y in 7 (9%), and N647I in 3 (4%). All mutations were located in exon 21, encoding the Src homology 2 (SH2) domain, which mediates the dimerization and activation of STAT protein. The amino acid changes resulted in a more hydrophobic protein surface and were associated with phosphorylation of STAT3 and its localization in the nucleus. In vitro functional studies showed that the Y640F and D661V mutations increased the transcriptional activity of STAT3. In the affected patients, downstream target genes of the STAT3 pathway (IFNGR2, BCL2L1, and JAK2) were up-regulated. Patients with STAT3 mutations presented more often with neutropenia and rheumatoid arthritis than did patients without these mutations.CONCLUSIONS: The SH2 dimerization and activation domain of STAT3 is frequently mutated in patients with large granular lymphocytic leukemia; these findings suggest that aberrant STAT3 signaling underlies the pathogenesis of this disease. (Funded by the Academy of Finland and others.).
KW - Aged
KW - Exome
KW - Gene Expression
KW - Humans
KW - Leukemia, Large Granular Lymphocytic/genetics
KW - Male
KW - Mutation
KW - Receptors, Antigen, T-Cell
KW - STAT3 Transcription Factor/genetics
KW - Sequence Analysis, RNA
KW - Transcription, Genetic
KW - Up-Regulation
U2 - 10.1056/NEJMoa1114885
DO - 10.1056/NEJMoa1114885
M3 - Journal article
C2 - 22591296
VL - 366
SP - 1905
EP - 1913
JO - New England Journal of Medicine
JF - New England Journal of Medicine
SN - 0028-4793
IS - 20
ER -
ID: 199432075