Soluble Collectin-12 (CL-12) Is a Pattern Recognition Molecule Initiating Complement Activation via the Alternative Pathway
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Soluble Collectin-12 (CL-12) Is a Pattern Recognition Molecule Initiating Complement Activation via the Alternative Pathway. / Ma, Ying Jie; Hein, Estrid; Munthe-Fog, Lea; Skjødt, Mikkel-Ole; Bayarri-Olmos, Rafael; Romani, Luigina; Garred, Peter.
In: Journal of immunology (Baltimore, Md. : 1950), Vol. 195, No. 7, 01.10.2015, p. 3365-73.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Soluble Collectin-12 (CL-12) Is a Pattern Recognition Molecule Initiating Complement Activation via the Alternative Pathway
AU - Ma, Ying Jie
AU - Hein, Estrid
AU - Munthe-Fog, Lea
AU - Skjødt, Mikkel-Ole
AU - Bayarri-Olmos, Rafael
AU - Romani, Luigina
AU - Garred, Peter
N1 - Copyright © 2015 by The American Association of Immunologists, Inc.
PY - 2015/10/1
Y1 - 2015/10/1
N2 - Soluble defense collagens including the collectins play important roles in innate immunity. Recently, a new member of the collectin family named collectin-12 (CL-12 or CL-P1) has been identified. CL-12 is highly expressed in umbilical cord vascular endothelial cells as a transmembrane receptor and may recognize certain bacteria and fungi, leading to opsonophagocytosis. However, based on its structural and functional similarities with soluble collectins, we hypothesized the existence of a fluid-phase analog of CL-12 released from cells, which may function as a soluble pattern-recognition molecule. Using recombinant CL-12 full length or CL-12 extracellular domain, we determined the occurrence of soluble CL-12 shed from in vitro cultured cells. Western blot showed that soluble recombinant CL-12 migrated with a band corresponding to ∼ 120 kDa under reducing conditions, whereas under nonreducing conditions it presented multimeric assembly forms. Immunoprecipitation and Western blot analysis of human umbilical cord plasma enabled identification of a natural soluble form of CL-12 having an electrophoretic mobility pattern close to that of shed soluble recombinant CL-12. Soluble CL-12 could recognize Aspergillus fumigatus partially through the carbohydrate-recognition domain in a Ca(2+)-independent manner. This led to activation of the alternative pathway of complement exclusively via association with properdin on A. fumigatus as validated by detection of C3b deposition and formation of the terminal complement complex. These results demonstrate the existence of CL-12 in a soluble form and indicate a novel mechanism by which the alternative pathway of complement may be triggered directly by a soluble pattern-recognition molecule.
AB - Soluble defense collagens including the collectins play important roles in innate immunity. Recently, a new member of the collectin family named collectin-12 (CL-12 or CL-P1) has been identified. CL-12 is highly expressed in umbilical cord vascular endothelial cells as a transmembrane receptor and may recognize certain bacteria and fungi, leading to opsonophagocytosis. However, based on its structural and functional similarities with soluble collectins, we hypothesized the existence of a fluid-phase analog of CL-12 released from cells, which may function as a soluble pattern-recognition molecule. Using recombinant CL-12 full length or CL-12 extracellular domain, we determined the occurrence of soluble CL-12 shed from in vitro cultured cells. Western blot showed that soluble recombinant CL-12 migrated with a band corresponding to ∼ 120 kDa under reducing conditions, whereas under nonreducing conditions it presented multimeric assembly forms. Immunoprecipitation and Western blot analysis of human umbilical cord plasma enabled identification of a natural soluble form of CL-12 having an electrophoretic mobility pattern close to that of shed soluble recombinant CL-12. Soluble CL-12 could recognize Aspergillus fumigatus partially through the carbohydrate-recognition domain in a Ca(2+)-independent manner. This led to activation of the alternative pathway of complement exclusively via association with properdin on A. fumigatus as validated by detection of C3b deposition and formation of the terminal complement complex. These results demonstrate the existence of CL-12 in a soluble form and indicate a novel mechanism by which the alternative pathway of complement may be triggered directly by a soluble pattern-recognition molecule.
KW - Aspergillus fumigatus
KW - Collectins
KW - Complement Activation
KW - Complement C3b
KW - Complement Pathway, Alternative
KW - Female
KW - Fetal Blood
KW - Human Umbilical Vein Endothelial Cells
KW - Humans
KW - Immunity, Innate
KW - Properdin
KW - Receptors, Scavenger
KW - Spores, Fungal
U2 - 10.4049/jimmunol.1500493
DO - 10.4049/jimmunol.1500493
M3 - Journal article
C2 - 26290605
VL - 195
SP - 3365
EP - 3373
JO - Journal of Immunology
JF - Journal of Immunology
SN - 0022-1767
IS - 7
ER -
ID: 161277117