Single-nucleus transcriptomics identifies separate classes of UCP1 and futile cycle adipocytes
Research output: Contribution to journal › Journal article › Research › peer-review
Standard
Single-nucleus transcriptomics identifies separate classes of UCP1 and futile cycle adipocytes. / Wang, Tongtong; Sharma, Anand Kumar; Wu, Chunyan; Maushart, Claudia Irene; Ghosh, Adhideb; Yang, Wu; Stefanicka, Patrik; Kovanicova, Zuzana; Ukropec, Jozef; Zhang, Jing; Arnold, Myrtha; Klug, Manuel; De Bock, Katrien; Schneider, Ulrich; Popescu, Cristina; Zheng, Bo; Ding, Lianggong; Long, Fen; Dewal, Revati Sumukh; Moser, Caroline; Sun, Wenfei; Dong, Hua; Takes, Martin; Suelberg, Dominique; Mameghani, Alexander; Nocito, Antonio; Zech, Christoph Johannes; Chirindel, Alin; Wild, Damian; Burger, Irene A; Schön, Michael R; Dietrich, Arne; Gao, Min; Heine, Markus; Sun, Yizhi; Vargas-Castillo, Ariana; Søberg, Susanna; Scheele, Camilla; Balaz, Miroslav; Blüher, Matthias; Betz, Matthias Johannes; Spiegelman, Bruce M; Wolfrum, Christian.
In: Cell Metabolism, 2024.Research output: Contribution to journal › Journal article › Research › peer-review
Harvard
APA
Vancouver
Author
Bibtex
}
RIS
TY - JOUR
T1 - Single-nucleus transcriptomics identifies separate classes of UCP1 and futile cycle adipocytes
AU - Wang, Tongtong
AU - Sharma, Anand Kumar
AU - Wu, Chunyan
AU - Maushart, Claudia Irene
AU - Ghosh, Adhideb
AU - Yang, Wu
AU - Stefanicka, Patrik
AU - Kovanicova, Zuzana
AU - Ukropec, Jozef
AU - Zhang, Jing
AU - Arnold, Myrtha
AU - Klug, Manuel
AU - De Bock, Katrien
AU - Schneider, Ulrich
AU - Popescu, Cristina
AU - Zheng, Bo
AU - Ding, Lianggong
AU - Long, Fen
AU - Dewal, Revati Sumukh
AU - Moser, Caroline
AU - Sun, Wenfei
AU - Dong, Hua
AU - Takes, Martin
AU - Suelberg, Dominique
AU - Mameghani, Alexander
AU - Nocito, Antonio
AU - Zech, Christoph Johannes
AU - Chirindel, Alin
AU - Wild, Damian
AU - Burger, Irene A
AU - Schön, Michael R
AU - Dietrich, Arne
AU - Gao, Min
AU - Heine, Markus
AU - Sun, Yizhi
AU - Vargas-Castillo, Ariana
AU - Søberg, Susanna
AU - Scheele, Camilla
AU - Balaz, Miroslav
AU - Blüher, Matthias
AU - Betz, Matthias Johannes
AU - Spiegelman, Bruce M
AU - Wolfrum, Christian
N1 - Copyright © 2024 The Author(s). Published by Elsevier Inc. All rights reserved.
PY - 2024
Y1 - 2024
N2 - Adipose tissue can recruit catabolic adipocytes that utilize chemical energy to dissipate heat. This process occurs either by uncoupled respiration through uncoupling protein 1 (UCP1) or by utilizing ATP-dependent futile cycles (FCs). However, it remains unclear how these pathways coexist since both processes rely on the mitochondrial membrane potential. Utilizing single-nucleus RNA sequencing to deconvolute the heterogeneity of subcutaneous adipose tissue in mice and humans, we identify at least 2 distinct subpopulations of beige adipocytes: FC-adipocytes and UCP1-beige adipocytes. Importantly, we demonstrate that the FC-adipocyte subpopulation is highly metabolically active and utilizes FCs to dissipate energy, thus contributing to thermogenesis independent of Ucp1. Furthermore, FC-adipocytes are important drivers of systemic energy homeostasis and linked to glucose metabolism and obesity resistance in humans. Taken together, our findings identify a noncanonical thermogenic adipocyte subpopulation, which could be an important regulator of energy homeostasis in mammals.
AB - Adipose tissue can recruit catabolic adipocytes that utilize chemical energy to dissipate heat. This process occurs either by uncoupled respiration through uncoupling protein 1 (UCP1) or by utilizing ATP-dependent futile cycles (FCs). However, it remains unclear how these pathways coexist since both processes rely on the mitochondrial membrane potential. Utilizing single-nucleus RNA sequencing to deconvolute the heterogeneity of subcutaneous adipose tissue in mice and humans, we identify at least 2 distinct subpopulations of beige adipocytes: FC-adipocytes and UCP1-beige adipocytes. Importantly, we demonstrate that the FC-adipocyte subpopulation is highly metabolically active and utilizes FCs to dissipate energy, thus contributing to thermogenesis independent of Ucp1. Furthermore, FC-adipocytes are important drivers of systemic energy homeostasis and linked to glucose metabolism and obesity resistance in humans. Taken together, our findings identify a noncanonical thermogenic adipocyte subpopulation, which could be an important regulator of energy homeostasis in mammals.
U2 - 10.1016/j.cmet.2024.07.005
DO - 10.1016/j.cmet.2024.07.005
M3 - Journal article
C2 - 39084216
JO - Cell Metabolism
JF - Cell Metabolism
SN - 1550-4131
ER -
ID: 400308101