Single-cell sorting of Plasmodium falciparum-infected erythrocytes expressing particular PfEMP1 variants

Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review

Standard

Single-cell sorting of Plasmodium falciparum-infected erythrocytes expressing particular PfEMP1 variants. / Lopez-Perez, Mary.

Malaria Immunology: Targeting the Surface of Infected Erythrocytes. Vol. 2470 Humana Press, 2022. p. 79-90 (Methods in molecular biology (Clifton, N.J.)).

Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review

Harvard

Lopez-Perez, M 2022, Single-cell sorting of Plasmodium falciparum-infected erythrocytes expressing particular PfEMP1 variants. in Malaria Immunology: Targeting the Surface of Infected Erythrocytes. vol. 2470, Humana Press, Methods in molecular biology (Clifton, N.J.), pp. 79-90. https://doi.org/10.1007/978-1-0716-2189-9_8

APA

Lopez-Perez, M. (2022). Single-cell sorting of Plasmodium falciparum-infected erythrocytes expressing particular PfEMP1 variants. In Malaria Immunology: Targeting the Surface of Infected Erythrocytes (Vol. 2470, pp. 79-90). Humana Press. Methods in molecular biology (Clifton, N.J.) https://doi.org/10.1007/978-1-0716-2189-9_8

Vancouver

Lopez-Perez M. Single-cell sorting of Plasmodium falciparum-infected erythrocytes expressing particular PfEMP1 variants. In Malaria Immunology: Targeting the Surface of Infected Erythrocytes. Vol. 2470. Humana Press. 2022. p. 79-90. (Methods in molecular biology (Clifton, N.J.)). https://doi.org/10.1007/978-1-0716-2189-9_8

Author

Lopez-Perez, Mary. / Single-cell sorting of Plasmodium falciparum-infected erythrocytes expressing particular PfEMP1 variants. Malaria Immunology: Targeting the Surface of Infected Erythrocytes. Vol. 2470 Humana Press, 2022. pp. 79-90 (Methods in molecular biology (Clifton, N.J.)).

Bibtex

@inbook{b65cb93923b14d9f9d6dddb440f967a1,
title = "Single-cell sorting of Plasmodium falciparum-infected erythrocytes expressing particular PfEMP1 variants",
abstract = "Cultures of Plasmodium falciparum often contain a heterogeneous parasite population. However, several studies require analysis of single infected erythrocytes (IEs) or a clonal parasite population derived from a single parasite. This protocol describes an efficient method for cloning by using fluorescence-activated cell sorting (FACS). For this, an antibody for a particular IEs surface protein it is added to the cell mixture to separate positive and negative IEs for that marker. After the separation, the viable homogeneous population can be used to grow in culture or for molecular analysis.",
keywords = "Animals, Erythrocytes/metabolism, Humans, Malaria, Falciparum/parasitology, Parasites/metabolism, Plasmodium falciparum/metabolism, Protozoan Proteins/metabolism",
author = "Mary Lopez-Perez",
note = "{\textcopyright} 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.",
year = "2022",
doi = "10.1007/978-1-0716-2189-9_8",
language = "English",
volume = "2470",
series = "Methods in molecular biology (Clifton, N.J.)",
publisher = "Humana Press",
pages = "79--90",
booktitle = "Malaria Immunology",
address = "United States",

}

RIS

TY - CHAP

T1 - Single-cell sorting of Plasmodium falciparum-infected erythrocytes expressing particular PfEMP1 variants

AU - Lopez-Perez, Mary

N1 - © 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

PY - 2022

Y1 - 2022

N2 - Cultures of Plasmodium falciparum often contain a heterogeneous parasite population. However, several studies require analysis of single infected erythrocytes (IEs) or a clonal parasite population derived from a single parasite. This protocol describes an efficient method for cloning by using fluorescence-activated cell sorting (FACS). For this, an antibody for a particular IEs surface protein it is added to the cell mixture to separate positive and negative IEs for that marker. After the separation, the viable homogeneous population can be used to grow in culture or for molecular analysis.

AB - Cultures of Plasmodium falciparum often contain a heterogeneous parasite population. However, several studies require analysis of single infected erythrocytes (IEs) or a clonal parasite population derived from a single parasite. This protocol describes an efficient method for cloning by using fluorescence-activated cell sorting (FACS). For this, an antibody for a particular IEs surface protein it is added to the cell mixture to separate positive and negative IEs for that marker. After the separation, the viable homogeneous population can be used to grow in culture or for molecular analysis.

KW - Animals

KW - Erythrocytes/metabolism

KW - Humans

KW - Malaria, Falciparum/parasitology

KW - Parasites/metabolism

KW - Plasmodium falciparum/metabolism

KW - Protozoan Proteins/metabolism

U2 - 10.1007/978-1-0716-2189-9_8

DO - 10.1007/978-1-0716-2189-9_8

M3 - Book chapter

C2 - 35881340

VL - 2470

T3 - Methods in molecular biology (Clifton, N.J.)

SP - 79

EP - 90

BT - Malaria Immunology

PB - Humana Press

ER -

ID: 320649585