Standard
Single-cell m6A mapping in vivo using picoMeRIP–seq. / Li, Yanjiao; Wang, Yunhao; Vera-Rodriguez, Maria; Lindeman, Leif Christopher; Skuggen, Linda Ellevog; Rasmussen, Erik M.K.; Jermstad, Ingunn; Khan, Shaista; Fosslie, Madeleine; Skuland, Trine; Indahl, Marie; Khodeer, Sherif; Klemsdal, Eva Kristine; Jin, Kang Xuan; Dalen, Knut Tomas; Fedorcsak, Peter; Greggains, Gareth D.; Lerdrup, Mads; Klungland, Arne; Au, Kin Fai; Dahl, John Arne.
In:
Nature Biotechnology, Vol. 42, 2024, p. 591-596.
Research output: Contribution to journal › Journal article › Research › peer-review
Harvard
Li, Y, Wang, Y, Vera-Rodriguez, M, Lindeman, LC, Skuggen, LE, Rasmussen, EMK, Jermstad, I, Khan, S, Fosslie, M, Skuland, T, Indahl, M, Khodeer, S, Klemsdal, EK, Jin, KX, Dalen, KT, Fedorcsak, P, Greggains, GD
, Lerdrup, M, Klungland, A, Au, KF & Dahl, JA 2024, '
Single-cell m6A mapping in vivo using picoMeRIP–seq',
Nature Biotechnology, vol. 42, pp. 591-596.
https://doi.org/10.1038/s41587-023-01831-7APA
Li, Y., Wang, Y., Vera-Rodriguez, M., Lindeman, L. C., Skuggen, L. E., Rasmussen, E. M. K., Jermstad, I., Khan, S., Fosslie, M., Skuland, T., Indahl, M., Khodeer, S., Klemsdal, E. K., Jin, K. X., Dalen, K. T., Fedorcsak, P., Greggains, G. D.
, Lerdrup, M., Klungland, A., ... Dahl, J. A. (2024).
Single-cell m6A mapping in vivo using picoMeRIP–seq.
Nature Biotechnology,
42, 591-596.
https://doi.org/10.1038/s41587-023-01831-7Vancouver
Li Y, Wang Y, Vera-Rodriguez M, Lindeman LC, Skuggen LE, Rasmussen EMK et al.
Single-cell m6A mapping in vivo using picoMeRIP–seq.
Nature Biotechnology. 2024;42:591-596.
https://doi.org/10.1038/s41587-023-01831-7Author
Li, Yanjiao ; Wang, Yunhao ; Vera-Rodriguez, Maria ; Lindeman, Leif Christopher ; Skuggen, Linda Ellevog ; Rasmussen, Erik M.K. ; Jermstad, Ingunn ; Khan, Shaista ; Fosslie, Madeleine ; Skuland, Trine ; Indahl, Marie ; Khodeer, Sherif ; Klemsdal, Eva Kristine ; Jin, Kang Xuan ; Dalen, Knut Tomas ; Fedorcsak, Peter ; Greggains, Gareth D. ; Lerdrup, Mads ; Klungland, Arne ; Au, Kin Fai ; Dahl, John Arne. / Single-cell m6A mapping in vivo using picoMeRIP–seq. In: Nature Biotechnology. 2024 ; Vol. 42. pp. 591-596.
Bibtex
@article{eb6b96b900944036a3fb92729d1f8cd7,
title = "Single-cell m6A mapping in vivo using picoMeRIP–seq",
abstract = "Current N 6-methyladenosine (m6A) mapping methods need large amounts of RNA or are limited to cultured cells. Through optimized sample recovery and signal-to-noise ratio, we developed picogram-scale m6A RNA immunoprecipitation and sequencing (picoMeRIP–seq) for studying m6A in vivo in single cells and scarce cell types using standard laboratory equipment. We benchmark m6A mapping on titrations of poly(A) RNA and embryonic stem cells and in single zebrafish zygotes, mouse oocytes and embryos.",
author = "Yanjiao Li and Yunhao Wang and Maria Vera-Rodriguez and Lindeman, {Leif Christopher} and Skuggen, {Linda Ellevog} and Rasmussen, {Erik M.K.} and Ingunn Jermstad and Shaista Khan and Madeleine Fosslie and Trine Skuland and Marie Indahl and Sherif Khodeer and Klemsdal, {Eva Kristine} and Jin, {Kang Xuan} and Dalen, {Knut Tomas} and Peter Fedorcsak and Greggains, {Gareth D.} and Mads Lerdrup and Arne Klungland and Au, {Kin Fai} and Dahl, {John Arne}",
note = "Publisher Copyright: {\textcopyright} 2023, The Author(s).",
year = "2024",
doi = "10.1038/s41587-023-01831-7",
language = "English",
volume = "42",
pages = "591--596",
journal = "Nature Biotechnology",
issn = "1087-0156",
publisher = "nature publishing group",
}
RIS
TY - JOUR
T1 - Single-cell m6A mapping in vivo using picoMeRIP–seq
AU - Li, Yanjiao
AU - Wang, Yunhao
AU - Vera-Rodriguez, Maria
AU - Lindeman, Leif Christopher
AU - Skuggen, Linda Ellevog
AU - Rasmussen, Erik M.K.
AU - Jermstad, Ingunn
AU - Khan, Shaista
AU - Fosslie, Madeleine
AU - Skuland, Trine
AU - Indahl, Marie
AU - Khodeer, Sherif
AU - Klemsdal, Eva Kristine
AU - Jin, Kang Xuan
AU - Dalen, Knut Tomas
AU - Fedorcsak, Peter
AU - Greggains, Gareth D.
AU - Lerdrup, Mads
AU - Klungland, Arne
AU - Au, Kin Fai
AU - Dahl, John Arne
N1 - Publisher Copyright:
© 2023, The Author(s).
PY - 2024
Y1 - 2024
N2 - Current N 6-methyladenosine (m6A) mapping methods need large amounts of RNA or are limited to cultured cells. Through optimized sample recovery and signal-to-noise ratio, we developed picogram-scale m6A RNA immunoprecipitation and sequencing (picoMeRIP–seq) for studying m6A in vivo in single cells and scarce cell types using standard laboratory equipment. We benchmark m6A mapping on titrations of poly(A) RNA and embryonic stem cells and in single zebrafish zygotes, mouse oocytes and embryos.
AB - Current N 6-methyladenosine (m6A) mapping methods need large amounts of RNA or are limited to cultured cells. Through optimized sample recovery and signal-to-noise ratio, we developed picogram-scale m6A RNA immunoprecipitation and sequencing (picoMeRIP–seq) for studying m6A in vivo in single cells and scarce cell types using standard laboratory equipment. We benchmark m6A mapping on titrations of poly(A) RNA and embryonic stem cells and in single zebrafish zygotes, mouse oocytes and embryos.
U2 - 10.1038/s41587-023-01831-7
DO - 10.1038/s41587-023-01831-7
M3 - Journal article
C2 - 37349523
AN - SCOPUS:85163048268
VL - 42
SP - 591
EP - 596
JO - Nature Biotechnology
JF - Nature Biotechnology
SN - 1087-0156
ER -
