Single-cell analysis pinpoints distinct populations of cytotoxic CD4+ T cells and an IL-10+CD109+ TH2 cell population in nasal polyps
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Single-cell analysis pinpoints distinct populations of cytotoxic CD4+ T cells and an IL-10+CD109+ TH2 cell population in nasal polyps. / Ma, Junjie; Tibbitt, Christopher A; Georén, Susanna Kumlien; Christian, Murray; Murrell, Ben; Cardell, Lars-Olaf; Bachert, Claus; Coquet, Jonathan M.
In: Science immunology, Vol. 6, No. 62, 13.08.2021.Research output: Contribution to journal › Journal article › Research › peer-review
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T1 - Single-cell analysis pinpoints distinct populations of cytotoxic CD4+ T cells and an IL-10+CD109+ TH2 cell population in nasal polyps
AU - Ma, Junjie
AU - Tibbitt, Christopher A
AU - Georén, Susanna Kumlien
AU - Christian, Murray
AU - Murrell, Ben
AU - Cardell, Lars-Olaf
AU - Bachert, Claus
AU - Coquet, Jonathan M
N1 - Copyright © 2021 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works.
PY - 2021/8/13
Y1 - 2021/8/13
N2 - Chronic rhinosinusitis with nasal polyps (CRSwNP) is characterized by a chronic inflammatory process often associated with comorbid asthma. In this study, we analyzed the transcriptomes of single T helper (TH) cells from nasal polyps of patients with CRSwNP and validated these findings using multiparameter flow cytometry. Polyp tissue contained suppressive T regulatory (Treg) cells, TH2 cells, type 2 innate lymphoid cells, and three transcriptionally distinct subsets of cytotoxic CD4+ T cells (CD4+ CTL). GATA3 expression was a feature of polyp Treg cells, whereas TH2 cells highly expressed TCN1, CD200R, and HPGDS and were enriched for genes involved in lipid metabolism. Only a portion of polyp TH2 cells expressed the prostaglandin D2 receptor CRTH2, whereas a subpopulation of CD109+CRTH2- TH2 cells expressed mRNA for common inhibitor receptors including LAG3 and TIM3 and produced IL-10. Together, we resolved the complexity of TH cells in patients with CRSwNP, identifying several distinct clusters of CD4+ CTL and a population of CD109+CRTH2- TH2 cells with putative regulatory potential.
AB - Chronic rhinosinusitis with nasal polyps (CRSwNP) is characterized by a chronic inflammatory process often associated with comorbid asthma. In this study, we analyzed the transcriptomes of single T helper (TH) cells from nasal polyps of patients with CRSwNP and validated these findings using multiparameter flow cytometry. Polyp tissue contained suppressive T regulatory (Treg) cells, TH2 cells, type 2 innate lymphoid cells, and three transcriptionally distinct subsets of cytotoxic CD4+ T cells (CD4+ CTL). GATA3 expression was a feature of polyp Treg cells, whereas TH2 cells highly expressed TCN1, CD200R, and HPGDS and were enriched for genes involved in lipid metabolism. Only a portion of polyp TH2 cells expressed the prostaglandin D2 receptor CRTH2, whereas a subpopulation of CD109+CRTH2- TH2 cells expressed mRNA for common inhibitor receptors including LAG3 and TIM3 and produced IL-10. Together, we resolved the complexity of TH cells in patients with CRSwNP, identifying several distinct clusters of CD4+ CTL and a population of CD109+CRTH2- TH2 cells with putative regulatory potential.
KW - Antigens, CD/immunology
KW - CD4-Positive T-Lymphocytes/immunology
KW - GPI-Linked Proteins/immunology
KW - Humans
KW - Interleukin-10/immunology
KW - Nasal Polyps/immunology
KW - Neoplasm Proteins/immunology
KW - Single-Cell Analysis
KW - T-Lymphocytes, Cytotoxic/immunology
KW - Th2 Cells/immunology
U2 - 10.1126/sciimmunol.abg6356
DO - 10.1126/sciimmunol.abg6356
M3 - Journal article
C2 - 34389612
VL - 6
JO - Advances in Immunology
JF - Advances in Immunology
SN - 0065-2776
IS - 62
ER -
ID: 356968049