TY - JOUR

T1 - Simple mucins (T, sialosyl-T, Tn and sialosyl-Tn) are not diagnostic for malignant breast lesions

AU - Reed, W

AU - Bryne, M

AU - Clausen, H

AU - Dabelsteen, Erik

AU - Nesland, J M

PY - 1994/3/1

Y1 - 1994/3/1

N2 - Immunohistochemical study of the distribution of carbohydrate core-structures on O-linked glycoproteins (T, sialosyl-T, Tn and sialosyl-Tn) was performed using specific monoclonal antibodies on 148 primary breast lesions, including 10 normal breast tissues, 16 benign lesions and 122 invasive carcinomas (79 localized and 43 metastatic lesions). T antigen, not observed in normal breast tissue, was present in 31% of the benign lesions and in some cases of morphologically normal epithelium adjacent to tumor cells, compatible with altered glycosylation being an early event. Sialosyl-T (s-T) antigen was present in all cases of normal epithelium and in 81% of the benign lesions. Both Tn and sialosyl-Tn (s-Tn) antigen were present in normal breast lesions. Both Tn and sialosyl-Tn (s-Tn) antigen were present in normal breast tissue (30%) and benign lesions (31% and 19%). In the malignant lesions, 20% were positive for T antigen, 82% for s-T antigen, 66% for Tn antigen and 22% for s-Tn antigen. The staining pattern was nearly identical for carcinomas with and without lymph node metastases. In conclusion, immunostaining for simple mucins does not permit a clear distinction between benign and malignant breast lesions.

AB - Immunohistochemical study of the distribution of carbohydrate core-structures on O-linked glycoproteins (T, sialosyl-T, Tn and sialosyl-Tn) was performed using specific monoclonal antibodies on 148 primary breast lesions, including 10 normal breast tissues, 16 benign lesions and 122 invasive carcinomas (79 localized and 43 metastatic lesions). T antigen, not observed in normal breast tissue, was present in 31% of the benign lesions and in some cases of morphologically normal epithelium adjacent to tumor cells, compatible with altered glycosylation being an early event. Sialosyl-T (s-T) antigen was present in all cases of normal epithelium and in 81% of the benign lesions. Both Tn and sialosyl-Tn (s-Tn) antigen were present in normal breast lesions. Both Tn and sialosyl-Tn (s-Tn) antigen were present in normal breast tissue (30%) and benign lesions (31% and 19%). In the malignant lesions, 20% were positive for T antigen, 82% for s-T antigen, 66% for Tn antigen and 22% for s-Tn antigen. The staining pattern was nearly identical for carcinomas with and without lymph node metastases. In conclusion, immunostaining for simple mucins does not permit a clear distinction between benign and malignant breast lesions.

KW - Antibodies, Monoclonal

KW - Antigens, Tumor-Associated, Carbohydrate

KW - Antigens, Viral, Tumor

KW - Biological Markers

KW - Breast

KW - Breast Neoplasms

KW - Carbohydrate Sequence

KW - Carbohydrates

KW - Carcinoma, Ductal, Breast

KW - Epithelial Cells

KW - Epithelium

KW - Epitopes

KW - Female

KW - Humans

KW - Immunohistochemistry

KW - Molecular Sequence Data

KW - Mucins

KW - Neoplasm Invasiveness

KW - Neoplasm Metastasis

KW - Oligosaccharides

KW - Tumor Markers, Biological

M3 - Journal article

C2 - 7516636

VL - 14

SP - 609

EP - 615

JO - Anticancer Research

JF - Anticancer Research

SN - 0250-7005

IS - 2B

ER -