Sex hormone binding globulin phenotypes: their detection and distribution in healthy adults and in different clinical conditions
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Sex hormone binding globulin phenotypes : their detection and distribution in healthy adults and in different clinical conditions. / Cornelissea, Mette M; Bennetta, Paul E; Christiansen, Michael; Blaakaer, Jan; Gluud, Christian; Andersen, Jens Rikardt; Homann, Christian; Garred, Peter.
In: Clinica Chimica Acta, Vol. 225, No. 2, 1994, p. 115-121.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Sex hormone binding globulin phenotypes
T2 - their detection and distribution in healthy adults and in different clinical conditions
AU - Cornelissea, Mette M
AU - Bennetta, Paul E
AU - Christiansen, Michael
AU - Blaakaer, Jan
AU - Gluud, Christian
AU - Andersen, Jens Rikardt
AU - Homann, Christian
AU - Garred, Peter
N1 - (Ekstern)
PY - 1994
Y1 - 1994
N2 - Human sex hormone binding globulin (SHBG) is encoded by a normal and a variant allele. The resulting SHBG phenotypes (the homozygous normal SHBG, the heterozygous SHBG and the homozygous variant SHBG phenotype) can be distinguished by their electrophoretic patterns. We developed a novel detection system allowing us to distinguish between the different electrophoretic patterns in small amounts of plasma or serum (10 μ1). Small aliquots of Blue Sepharose were added to diluted sera or plasma samples for removal of albumin and the supernatants were subsequently applied to Western blotting. This method of detection was used to determine the distribution of SHBG phenotypes in healthy controls of both sexes and in five different pathological conditions characterized by changes in the SHBG level or endocrine disturbances (malignant and benign ovarian neoplasms, hirsutism, liver cirrhosis and alcoholism). The distribution of SHBG phenotypes was independent of such conditions and in agreement with the expected phenotype distribution of a bi-allelic gene in both healthy controls and patients (Hardy Weinberg law). An allele frequency of 0.13 was found for the SHBG variant allele based on the experimental values. Differences in SHBG phenotypes do not appear to have any clinical significance and no sex difference was found in the SHBG phenotype distribution.
AB - Human sex hormone binding globulin (SHBG) is encoded by a normal and a variant allele. The resulting SHBG phenotypes (the homozygous normal SHBG, the heterozygous SHBG and the homozygous variant SHBG phenotype) can be distinguished by their electrophoretic patterns. We developed a novel detection system allowing us to distinguish between the different electrophoretic patterns in small amounts of plasma or serum (10 μ1). Small aliquots of Blue Sepharose were added to diluted sera or plasma samples for removal of albumin and the supernatants were subsequently applied to Western blotting. This method of detection was used to determine the distribution of SHBG phenotypes in healthy controls of both sexes and in five different pathological conditions characterized by changes in the SHBG level or endocrine disturbances (malignant and benign ovarian neoplasms, hirsutism, liver cirrhosis and alcoholism). The distribution of SHBG phenotypes was independent of such conditions and in agreement with the expected phenotype distribution of a bi-allelic gene in both healthy controls and patients (Hardy Weinberg law). An allele frequency of 0.13 was found for the SHBG variant allele based on the experimental values. Differences in SHBG phenotypes do not appear to have any clinical significance and no sex difference was found in the SHBG phenotype distribution.
KW - Alcoholism
KW - Carbohydrate heterogeneity
KW - Electrophoresis
KW - Hirsutism
KW - Liver cirrhosis
KW - Ovarian tumor
KW - Sex hormone binding globulin phenotypes
UR - http://www.scopus.com/inward/record.url?scp=0028243833&partnerID=8YFLogxK
U2 - 10.1016/0009-8981(94)90039-6
DO - 10.1016/0009-8981(94)90039-6
M3 - Journal article
C2 - 8088001
AN - SCOPUS:0028243833
VL - 225
SP - 115
EP - 121
JO - Clinica Chimica Acta
JF - Clinica Chimica Acta
SN - 0009-8981
IS - 2
ER -
ID: 251989395