Sequenced response of extracellular matrix deadhesion and fibrotic regulators after muscle damage is involved in protection against future injury in human skeletal muscle
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Sequenced response of extracellular matrix deadhesion and fibrotic regulators after muscle damage is involved in protection against future injury in human skeletal muscle. / Mackey, Abigail; Brandstetter, Simon; Schjerling, Peter; Bojsen-Moller, Jens; Qvortrup, Klaus; Pedersen, Mette M; Doessing, Simon; Kjær, Michael; Magnusson, S Peter; Langberg, Henning.
In: FASEB Journal, Vol. 25, No. 6, 2011, p. 1943-1959.Research output: Contribution to journal › Journal article › Research › peer-review
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T1 - Sequenced response of extracellular matrix deadhesion and fibrotic regulators after muscle damage is involved in protection against future injury in human skeletal muscle
AU - Mackey, Abigail
AU - Brandstetter, Simon
AU - Schjerling, Peter
AU - Bojsen-Moller, Jens
AU - Qvortrup, Klaus
AU - Pedersen, Mette M
AU - Doessing, Simon
AU - Kjær, Michael
AU - Magnusson, S Peter
AU - Langberg, Henning
PY - 2011
Y1 - 2011
N2 - The purpose of this study was to test the hypothesis that remodeling of skeletal muscle extracellular matrix (ECM) is involved in protecting human muscle against injury. Biopsies were obtained from medial gastrocnemius muscles after a single bout of electrical stimulation (B) or a repeated bout (RB) 30 d later, or 30 d after a single stimulation bout (RBc). A muscle biopsy was collected from the control leg for comparison with the stimulated leg. Satellite cell content, tenascin C, and muscle regeneration were assessed by immunohistochemistry; real-time PCR was used to measure mRNA levels of collagens, laminins, heat-shock proteins (HSPs), inflammation, and related growth factors. The large responses of HSPs, CCL2, and tenascin C detected 48 h after a single bout were attenuated in the RB trial, indicative of protection against injury. Satellite cell content and 12 target genes, including IGF-1, were elevated 30 d after a single bout. Among those displaying the greatest difference vs. control muscle, ECM laminin-ß1 and collagen types I and III were elevated ~6- to 9-fold (P
AB - The purpose of this study was to test the hypothesis that remodeling of skeletal muscle extracellular matrix (ECM) is involved in protecting human muscle against injury. Biopsies were obtained from medial gastrocnemius muscles after a single bout of electrical stimulation (B) or a repeated bout (RB) 30 d later, or 30 d after a single stimulation bout (RBc). A muscle biopsy was collected from the control leg for comparison with the stimulated leg. Satellite cell content, tenascin C, and muscle regeneration were assessed by immunohistochemistry; real-time PCR was used to measure mRNA levels of collagens, laminins, heat-shock proteins (HSPs), inflammation, and related growth factors. The large responses of HSPs, CCL2, and tenascin C detected 48 h after a single bout were attenuated in the RB trial, indicative of protection against injury. Satellite cell content and 12 target genes, including IGF-1, were elevated 30 d after a single bout. Among those displaying the greatest difference vs. control muscle, ECM laminin-ß1 and collagen types I and III were elevated ~6- to 9-fold (P
U2 - 10.1096/fj.10-176487
DO - 10.1096/fj.10-176487
M3 - Journal article
C2 - 21368102
VL - 25
SP - 1943
EP - 1959
JO - F A S E B Journal
JF - F A S E B Journal
SN - 0892-6638
IS - 6
ER -
ID: 33432514