Sensitization to lipopolysaccharide in mice with asymptomatic viral infection: role of T cell-dependent production of interferon-gamma
Research output: Contribution to journal › Journal article › Research › peer-review
The interplay between viral infection and lipopolysaccharide (LPS) was studied. Infection with a noncytopathogenic virus, lymphocytic choriomeningitis virus (LCMV), was found to sensitize mice to low doses of LPS. In vivo, this hypersensitivity correlated with hyperproduction of tumor necrosis factor-alpha (TNF-alpha), and in vitro, LPS-stimulated splenic adherent cells produced increased amounts of TNF-alpha. Hyperproduction of TNF-alpha was temporally correlated with virus-induced production of interferon-gamma (IFN-gamma); only marginally increased IFN-gamma and TNF-alpha production was observed in LCMV-infected, T cell-deficient mice and in mice infected with vesicular stomatitis virus, a virus that induces much less T cell activation than does LCMV. Finally, LCMV infection was much less efficient in priming IFN-gamma knockout mice for hyperproduction of TNF-alpha. These findings indicate that clinically silent viral infections may induce hypersensitivity to LPS through T cell activation and subsequent production of IFN-gamma; this sensitizes monocytes/macrophages for hyperproduction of TNF-alpha.
Original language | English |
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Journal | Journal of Infectious Diseases |
Volume | 176 |
Issue number | 1 |
Pages (from-to) | 151-7 |
Number of pages | 6 |
ISSN | 0022-1899 |
Publication status | Published - 1997 |
Bibliographical note
Keywords: Animals; Female; Interferon-gamma; Interleukin-10; Lipopolysaccharides; Lymphocytic Choriomeningitis; Mice; Mice, Inbred C57BL; Mice, Nude; T-Lymphocytes; Tumor Necrosis Factor-alpha
ID: 9639728