Secretory phospholipase A2-mediated neuronal cell death involves glutamate ionotropic receptors
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Secretory phospholipase A2-mediated neuronal cell death involves glutamate ionotropic receptors. / Kolko, Miriam; de Turco, Elena B; Diemer, Nils Henrik; Bazan, Nicolas G.
In: NeuroReport, Vol. 13, No. 15, 28.10.2002, p. 1963-1966.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Secretory phospholipase A2-mediated neuronal cell death involves glutamate ionotropic receptors
AU - Kolko, Miriam
AU - de Turco, Elena B
AU - Diemer, Nils Henrik
AU - Bazan, Nicolas G
PY - 2002/10/28
Y1 - 2002/10/28
N2 - To define the significance of glutamate ionotropic receptors in sPLA -mediated neuronal cell death we used the NMDA receptor antagonist MK-801 and the AMPA receptor antagonist PNQX. In primary neuronal cell cultures both MK-801 and PNQX inhibited sPLA - and glutamate-induced neuronal death. [ H]Arachidonic acid release induced by both sPLA and glutamate was partially blocked by MK-801, indicating that the glutamate-NMDA-cPLA pathway contributes to sPLA -induced arachidonic acid release. Systemic administration of MK-801 to rats that had sPLA injected into the right striatum significantly decreased neuronal cell death. We conclude that glutamatergic synaptic activity modulates sPLA -induced neuronal cell death.
AB - To define the significance of glutamate ionotropic receptors in sPLA -mediated neuronal cell death we used the NMDA receptor antagonist MK-801 and the AMPA receptor antagonist PNQX. In primary neuronal cell cultures both MK-801 and PNQX inhibited sPLA - and glutamate-induced neuronal death. [ H]Arachidonic acid release induced by both sPLA and glutamate was partially blocked by MK-801, indicating that the glutamate-NMDA-cPLA pathway contributes to sPLA -induced arachidonic acid release. Systemic administration of MK-801 to rats that had sPLA injected into the right striatum significantly decreased neuronal cell death. We conclude that glutamatergic synaptic activity modulates sPLA -induced neuronal cell death.
KW - Animals
KW - Arachidonic Acid
KW - Astrocytes
KW - Body Temperature
KW - Brain
KW - Cell Death
KW - Cells, Cultured
KW - Cerebral Infarction
KW - Dizocilpine Maleate
KW - Dose-Response Relationship, Drug
KW - Excitatory Amino Acid Antagonists
KW - Excitatory Amino Acids
KW - Fetus
KW - Group II Phospholipases A2
KW - Humans
KW - Neostriatum
KW - Neurons
KW - Phospholipases A
KW - Phospholipases A2
KW - Quinoxalines
KW - Rats
KW - Rats, Wistar
KW - Receptors, AMPA
KW - Receptors, N-Methyl-D-Aspartate
U2 - 10.1097/00001756-200210280-00026
DO - 10.1097/00001756-200210280-00026
M3 - Journal article
C2 - 12395100
VL - 13
SP - 1963
EP - 1966
JO - NeuroReport
JF - NeuroReport
SN - 0959-4965
IS - 15
ER -
ID: 128615058