Release of tensile strain on engineered human tendon tissue disturbs cell adhesions, changes matrix architecture, and induces an inflammatory phenotype
Research output: Contribution to journal › Journal article › Research › peer-review
Standard
Release of tensile strain on engineered human tendon tissue disturbs cell adhesions, changes matrix architecture, and induces an inflammatory phenotype. / Bayer, Monika L; Schjerling, Peter; Herchenhan, Andreas; Zeltz, Cedric; Heinemeier, Katja Maria; Christensen, Lise; Krogsgaard, Michael; Gullberg, Donald; Kjaer, Michael.
In: PLOS ONE, Vol. 9, No. 1, e86078, 2014, p. 1-16.Research output: Contribution to journal › Journal article › Research › peer-review
Harvard
APA
Vancouver
Author
Bibtex
}
RIS
TY - JOUR
T1 - Release of tensile strain on engineered human tendon tissue disturbs cell adhesions, changes matrix architecture, and induces an inflammatory phenotype
AU - Bayer, Monika L
AU - Schjerling, Peter
AU - Herchenhan, Andreas
AU - Zeltz, Cedric
AU - Heinemeier, Katja Maria
AU - Christensen, Lise
AU - Krogsgaard, Michael
AU - Gullberg, Donald
AU - Kjaer, Michael
PY - 2014
Y1 - 2014
N2 - Mechanical loading of tendon cells results in an upregulation of mechanotransduction signaling pathways, cell-matrix adhesion and collagen synthesis, but whether unloading removes these responses is unclear. We investigated the response to tension release, with regard to matrix proteins, pro-inflammatory mediators and tendon phenotypic specific molecules, in an in vitro model where tendon-like tissue was engineered from human tendon cells. Tissue sampling was performed 1, 2, 4 and 6 days after surgical de-tensioning of the tendon construct. When tensile stimulus was removed, integrin type collagen receptors showed a contrasting response with a clear drop in integrin subunit α11 mRNA and protein expression, and an increase in α2 integrin mRNA and protein levels. Further, specific markers for tendon cell differentiation declined and normal tendon architecture was disturbed, whereas pro-inflammatory molecules were upregulated. Stimulation with the cytokine TGF-β1 had distinct effects on some tendon-related genes in both tensioned and de-tensioned tissue. These findings indicate an important role of mechanical loading for cellular and matrix responses in tendon, including that loss of tension leads to a decrease in phenotypical markers for tendon, while expression of pro-inflammatory mediators is induced.
AB - Mechanical loading of tendon cells results in an upregulation of mechanotransduction signaling pathways, cell-matrix adhesion and collagen synthesis, but whether unloading removes these responses is unclear. We investigated the response to tension release, with regard to matrix proteins, pro-inflammatory mediators and tendon phenotypic specific molecules, in an in vitro model where tendon-like tissue was engineered from human tendon cells. Tissue sampling was performed 1, 2, 4 and 6 days after surgical de-tensioning of the tendon construct. When tensile stimulus was removed, integrin type collagen receptors showed a contrasting response with a clear drop in integrin subunit α11 mRNA and protein expression, and an increase in α2 integrin mRNA and protein levels. Further, specific markers for tendon cell differentiation declined and normal tendon architecture was disturbed, whereas pro-inflammatory molecules were upregulated. Stimulation with the cytokine TGF-β1 had distinct effects on some tendon-related genes in both tensioned and de-tensioned tissue. These findings indicate an important role of mechanical loading for cellular and matrix responses in tendon, including that loss of tension leads to a decrease in phenotypical markers for tendon, while expression of pro-inflammatory mediators is induced.
KW - Adolescent
KW - Adult
KW - Biological Markers
KW - Cell Adhesion
KW - Cellular Microenvironment
KW - Collagen
KW - Extracellular Matrix
KW - Gene Expression Regulation
KW - Humans
KW - Inflammation
KW - Inflammation Mediators
KW - Integrins
KW - Phenotype
KW - Protein Subunits
KW - RNA, Messenger
KW - Stress, Mechanical
KW - Tendons
KW - Tensile Strength
KW - Time Factors
KW - Tissue Culture Techniques
KW - Tissue Engineering
KW - Tissue Scaffolds
KW - Transforming Growth Factor beta1
KW - Young Adult
U2 - 10.1371/journal.pone.0086078
DO - 10.1371/journal.pone.0086078
M3 - Journal article
C2 - 24465881
VL - 9
SP - 1
EP - 16
JO - PLoS ONE
JF - PLoS ONE
SN - 1932-6203
IS - 1
M1 - e86078
ER -
ID: 127352539