Recruitment of SHP-1 protein tyrosine phosphatase and signalling by a chimeric T-cell receptor-killer inhibitory receptor
Research output: Contribution to journal › Journal article › Research › peer-review
Receptors expressing the immunoreceptor tyrosine-based inhibitory motif (ITIM) in their cytoplasmic tail play an important role in the negative regulation of natural killer and B-cell activation. A subpopulation of T cells expresses the ITIM containing killer cell inhibitory receptor (KIR), which recognize MHC class I molecules. Following coligation of KIR with an activating receptor, the tyrosine in the ITIM is phosphorylated and the cytoplasmic protein tyrosine phosphatase SHP-1 is recruited to the ITIM via its SH2 domains. It is still not clear how SHP-1 affects T-cell receptor (TCR) signalling. In this study, we constructed a chimeric TCR-KIR receptor. We demonstrated that SHP-1 is recruited to the chimeric TCR-KIR receptor following T-cell stimulation with either anti-TCR monoclonal antibody (MoAb) or superantigen. However, in spite of this we could not detect any effect of SHP-1 on TCR signalling regarding total protein tyrosine phosphorylation, TCR down-regulation, mobilization of intracellular free calcium, or induction of the activation markers CD69 and CD25.
Original language | English |
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Journal | Scandinavian Journal of Immunology |
Volume | 51 |
Issue number | 6 |
Pages (from-to) | 557-64 |
Number of pages | 7 |
ISSN | 0300-9475 |
Publication status | Published - 2000 |
Bibliographical note
Keywords: Amino Acid Sequence; Animals; Antigens, CD; Antigens, CD3; Antigens, Differentiation, T-Lymphocyte; Calcium; Cells, Cultured; Humans; Intracellular Fluid; Intracellular Signaling Peptides and Proteins; Killer Cells, Natural; Ligands; Mice; Molecular Sequence Data; Phosphorylation; Protein Tyrosine Phosphatase, Non-Receptor Type 6; Protein Tyrosine Phosphatases; Receptors, Antigen, T-Cell; Receptors, Cytoplasmic and Nuclear; Receptors, Immunologic; Receptors, Interleukin-2; Receptors, KIR; Recombinant Fusion Proteins; Signal Transduction; Tyrosine
ID: 8545051