Rationally Engineered Tandem Facial Amphiphiles for Improved Membrane Protein Stabilization Efficacy
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Rationally Engineered Tandem Facial Amphiphiles for Improved Membrane Protein Stabilization Efficacy. / Das, Manabendra; Du, Yang; Mortensen, Jonas S.; Hariharan, Parameswaran; Lee, Hyun Sung; Byrne, Bernadette; Loland, Claus J.; Guan, Lan; Kobilka, Brian K.; Chae, Pil Seok.
In: ChemBioChem, Vol. 19, No. 20, 2018, p. 2225-2232.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Rationally Engineered Tandem Facial Amphiphiles for Improved Membrane Protein Stabilization Efficacy
AU - Das, Manabendra
AU - Du, Yang
AU - Mortensen, Jonas S.
AU - Hariharan, Parameswaran
AU - Lee, Hyun Sung
AU - Byrne, Bernadette
AU - Loland, Claus J.
AU - Guan, Lan
AU - Kobilka, Brian K.
AU - Chae, Pil Seok
PY - 2018
Y1 - 2018
N2 - A new family of tandem facial glucosides/maltosides (TFGs/TFMs) for membrane protein manipulation was prepared. The best detergent varied depending on the hydrophobic thickness of the target protein, but ether-based TFMs (TFM-C0E, TFM-C3E, and TFM-C5E) were notable for their ability to confer higher membrane protein stability than the previously developed amide-based TFA-1 (P. S. Chae, K. Gotfryd, J. Pacyna, L. J. W. Miercke, S. G. F. Rasmussen, R. A. Robbins, R. R. Rana, C. J. Loland, B. Kobilka, R. Stroud, B. Byrne, U. Gether, S. H. Gellman, J. Am. Chem. Soc. 2010, 132, 16750–16752). Thus, this study not only introduces novel agents with the potential to be used in membrane protein research but also highlights the importance of both the hydrophobic length and linker functionality of the detergent in stabilizing membrane proteins.
AB - A new family of tandem facial glucosides/maltosides (TFGs/TFMs) for membrane protein manipulation was prepared. The best detergent varied depending on the hydrophobic thickness of the target protein, but ether-based TFMs (TFM-C0E, TFM-C3E, and TFM-C5E) were notable for their ability to confer higher membrane protein stability than the previously developed amide-based TFA-1 (P. S. Chae, K. Gotfryd, J. Pacyna, L. J. W. Miercke, S. G. F. Rasmussen, R. A. Robbins, R. R. Rana, C. J. Loland, B. Kobilka, R. Stroud, B. Byrne, U. Gether, S. H. Gellman, J. Am. Chem. Soc. 2010, 132, 16750–16752). Thus, this study not only introduces novel agents with the potential to be used in membrane protein research but also highlights the importance of both the hydrophobic length and linker functionality of the detergent in stabilizing membrane proteins.
KW - amphiphiles
KW - detergent design
KW - detergent faciality
KW - protein stabilization
KW - protein structures
U2 - 10.1002/cbic.201800388
DO - 10.1002/cbic.201800388
M3 - Journal article
C2 - 30070754
AN - SCOPUS:85053614521
VL - 19
SP - 2225
EP - 2232
JO - ChemBioChem
JF - ChemBioChem
SN - 1439-4227
IS - 20
ER -
ID: 209802023