Rad52 and Rad59 exhibit both overlapping and distinct functions
Research output: Contribution to journal › Journal article › Research › peer-review
Homologous recombination is an important pathway for the repair of DNA double-strand breaks (DSBs). In the yeast Saccharomyces cerevisiae, Rad52 is a central recombination protein, whereas its paralogue, Rad59, plays a more subtle role in homologous recombination. Both proteins can mediate annealing of complementary single-stranded DNA in vitro, but only Rad52 interacts with replication protein A and the Rad51 recombinase. We have studied the functional overlap between Rad52 and Rad59 in living cells using chimeras of the two proteins and site-directed mutagenesis. We find that Rad52 and Rad59 have both overlapping as well as separate functions in DSB repair. Importantly, the N-terminus of Rad52 possesses functions not supplied by Rad59, which may account for its central role in homologous recombination.
Original language | English |
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Journal | DNA Repair |
Volume | 6 |
Issue number | 1 |
Pages (from-to) | 27-37 |
ISSN | 1568-7864 |
DOIs | |
Publication status | Published - 2007 |
Bibliographical note
Keywords: Rad52; Rad59; Recombination foci; Homologous recombination; Saccharomyces cerevisiae
ID: 1095346