Quantifying spatial position in a branched structure in immunostained mouse tissue sections
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Quantifying spatial position in a branched structure in immunostained mouse tissue sections. / Heilmann, Silja; Semb, Tor Henrik; Nyeng, Pia.
In: STAR Protocols, Vol. 2, No. 4, 100806, 2021.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Quantifying spatial position in a branched structure in immunostained mouse tissue sections
AU - Heilmann, Silja
AU - Semb, Tor Henrik
AU - Nyeng, Pia
N1 - © 2021 The Author(s).
PY - 2021
Y1 - 2021
N2 - We have developed a protocol to quantify the position of a cell in a branched structure based on two-dimensional microscopy images of tissue sections. Biological branched structures include organs such as the lungs, kidneys, and pancreas. In these organs, cell fate has been correlated with position, based on a qualitative estimate. However, a quantitative means of evaluating the cell position has been lacking. With this protocol, the correlation between cell fate and cell position was measured in mouse embryonic pancreas. For complete details on the use and execution of this protocol, please refer to Nyeng et al. (2019).
AB - We have developed a protocol to quantify the position of a cell in a branched structure based on two-dimensional microscopy images of tissue sections. Biological branched structures include organs such as the lungs, kidneys, and pancreas. In these organs, cell fate has been correlated with position, based on a qualitative estimate. However, a quantitative means of evaluating the cell position has been lacking. With this protocol, the correlation between cell fate and cell position was measured in mouse embryonic pancreas. For complete details on the use and execution of this protocol, please refer to Nyeng et al. (2019).
U2 - 10.1016/j.xpro.2021.100806
DO - 10.1016/j.xpro.2021.100806
M3 - Journal article
C2 - 34632415
VL - 2
JO - STAR Protocols
JF - STAR Protocols
SN - 2666-1667
IS - 4
M1 - 100806
ER -
ID: 284836641