Protein localization screening in vivo reveals novel regulators of multiciliated cell development and function

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Protein localization screening in vivo reveals novel regulators of multiciliated cell development and function. / Tu, Fan; Sedzinski, Jakub; Ma, Yun; Marcotte, Edward M; Wallingford, John B.

In: Journal of Cell Science, Vol. 131, No. 3, 206565, 29.01.2018, p. 1-10.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Tu, F, Sedzinski, J, Ma, Y, Marcotte, EM & Wallingford, JB 2018, 'Protein localization screening in vivo reveals novel regulators of multiciliated cell development and function', Journal of Cell Science, vol. 131, no. 3, 206565, pp. 1-10. https://doi.org/10.1242/jcs.206565

APA

Tu, F., Sedzinski, J., Ma, Y., Marcotte, E. M., & Wallingford, J. B. (2018). Protein localization screening in vivo reveals novel regulators of multiciliated cell development and function. Journal of Cell Science, 131(3), 1-10. [206565]. https://doi.org/10.1242/jcs.206565

Vancouver

Tu F, Sedzinski J, Ma Y, Marcotte EM, Wallingford JB. Protein localization screening in vivo reveals novel regulators of multiciliated cell development and function. Journal of Cell Science. 2018 Jan 29;131(3):1-10. 206565. https://doi.org/10.1242/jcs.206565

Author

Tu, Fan ; Sedzinski, Jakub ; Ma, Yun ; Marcotte, Edward M ; Wallingford, John B. / Protein localization screening in vivo reveals novel regulators of multiciliated cell development and function. In: Journal of Cell Science. 2018 ; Vol. 131, No. 3. pp. 1-10.

Bibtex

@article{4b971ffa81264d01af5e96e1a2b079bd,

title = "Protein localization screening in vivo reveals novel regulators of multiciliated cell development and function",

abstract = "Multiciliated cells (MCCs) drive fluid flow in diverse tubular organs and are essential for the development and homeostasis of the vertebrate central nervous system, airway and reproductive tracts. These cells are characterized by dozens or hundreds of motile cilia that beat in a coordinated and polarized manner. In recent years, genomic studies have not only elucidated the transcriptional hierarchy for MCC specification but also identified myriad new proteins that govern MCC ciliogenesis, cilia beating and cilia polarization. Interestingly, this burst of genomic data has also highlighted that proteins with no obvious role in cilia do, in fact, have important ciliary functions. Understanding the function of proteins with little prior history of study presents a special challenge, especially when faced with large numbers of such proteins. Here, we define the subcellular localization in MCCs of ∼200 proteins not previously implicated in cilia biology. Functional analyses arising from the screen provide novel links between actin cytoskeleton and MCC ciliogenesis.",

author = "Fan Tu and Jakub Sedzinski and Yun Ma and Marcotte, {Edward M} and Wallingford, {John B}",

note = "{\textcopyright} 2018. Published by The Company of Biologists Ltd.",

year = "2018",

month = jan,

day = "29",

doi = "10.1242/jcs.206565",

language = "English",

volume = "131",

pages = "1--10",

journal = "Journal of Cell Science",

issn = "0021-9533",

publisher = "The/Company of Biologists Ltd.",

number = "3",

}

RIS

TY - JOUR

T1 - Protein localization screening in vivo reveals novel regulators of multiciliated cell development and function

AU - Tu, Fan

AU - Sedzinski, Jakub

AU - Ma, Yun

AU - Marcotte, Edward M

AU - Wallingford, John B

PY - 2018/1/29

Y1 - 2018/1/29

N2 - Multiciliated cells (MCCs) drive fluid flow in diverse tubular organs and are essential for the development and homeostasis of the vertebrate central nervous system, airway and reproductive tracts. These cells are characterized by dozens or hundreds of motile cilia that beat in a coordinated and polarized manner. In recent years, genomic studies have not only elucidated the transcriptional hierarchy for MCC specification but also identified myriad new proteins that govern MCC ciliogenesis, cilia beating and cilia polarization. Interestingly, this burst of genomic data has also highlighted that proteins with no obvious role in cilia do, in fact, have important ciliary functions. Understanding the function of proteins with little prior history of study presents a special challenge, especially when faced with large numbers of such proteins. Here, we define the subcellular localization in MCCs of ∼200 proteins not previously implicated in cilia biology. Functional analyses arising from the screen provide novel links between actin cytoskeleton and MCC ciliogenesis.

AB - Multiciliated cells (MCCs) drive fluid flow in diverse tubular organs and are essential for the development and homeostasis of the vertebrate central nervous system, airway and reproductive tracts. These cells are characterized by dozens or hundreds of motile cilia that beat in a coordinated and polarized manner. In recent years, genomic studies have not only elucidated the transcriptional hierarchy for MCC specification but also identified myriad new proteins that govern MCC ciliogenesis, cilia beating and cilia polarization. Interestingly, this burst of genomic data has also highlighted that proteins with no obvious role in cilia do, in fact, have important ciliary functions. Understanding the function of proteins with little prior history of study presents a special challenge, especially when faced with large numbers of such proteins. Here, we define the subcellular localization in MCCs of ∼200 proteins not previously implicated in cilia biology. Functional analyses arising from the screen provide novel links between actin cytoskeleton and MCC ciliogenesis.

U2 - 10.1242/jcs.206565

DO - 10.1242/jcs.206565

M3 - Journal article

C2 - 29180514

VL - 131

SP - 1

EP - 10

JO - Journal of Cell Science

JF - Journal of Cell Science

SN - 0021-9533

IS - 3

M1 - 206565

ER -

ID: 202029716

Forskning