Progastrin processing differs in 7B2 and PC2 knockout animals: A role for 7B2 independent of action on PC2
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Progastrin processing differs in 7B2 and PC2 knockout animals : A role for 7B2 independent of action on PC2. / Rehfeld, Jens F.; Lindberg, Iris; Friis-Hansen, Lennart.
In: FEBS Letters, Vol. 510, No. 1-2, 02.01.2002, p. 89-93.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Progastrin processing differs in 7B2 and PC2 knockout animals
T2 - A role for 7B2 independent of action on PC2
AU - Rehfeld, Jens F.
AU - Lindberg, Iris
AU - Friis-Hansen, Lennart
N1 - Funding Information: The skillful technical assistance of Rikke Grønholt and Alice von der Lieth is gratefully acknowledged. The study was supported by grants from the Danish Medical Research Council, the Danish Biotechnology Program for Peptide Research and Cellular Communication and NIH Grants (DA05084 and DK49703).
PY - 2002/1/2
Y1 - 2002/1/2
N2 - Cellular synthesis of neuroendocrine peptides requires prohormone convertases (PCs). In order to determine the role of PC2 for gastrin synthesis, we examined antral extracts from mice lacking PC2 or its chaperone, 7B2. The overall concentrations of precursors and α-amidated gastrins were similar in all mice. Chromatography, however, revealed that while the K53-K54 site was almost fully cleaved in controls and half cleaved in PC2 null mice, only 23% was cleaved in 7B2 null mice. The results show that PC2 and 7B2 both are required for synthesis of the main form of gastrin (gastrin-17), and that 7B2 exhibits effects beyond PC2-mediated cleavages.
AB - Cellular synthesis of neuroendocrine peptides requires prohormone convertases (PCs). In order to determine the role of PC2 for gastrin synthesis, we examined antral extracts from mice lacking PC2 or its chaperone, 7B2. The overall concentrations of precursors and α-amidated gastrins were similar in all mice. Chromatography, however, revealed that while the K53-K54 site was almost fully cleaved in controls and half cleaved in PC2 null mice, only 23% was cleaved in 7B2 null mice. The results show that PC2 and 7B2 both are required for synthesis of the main form of gastrin (gastrin-17), and that 7B2 exhibits effects beyond PC2-mediated cleavages.
KW - 7B2
KW - Chaperone
KW - Gastrin
KW - Progastrin
KW - Prohormone convertase
KW - Prohormone processing
UR - http://www.scopus.com/inward/record.url?scp=0037005995&partnerID=8YFLogxK
U2 - 10.1016/S0014-5793(01)03234-3
DO - 10.1016/S0014-5793(01)03234-3
M3 - Journal article
C2 - 11755537
AN - SCOPUS:0037005995
VL - 510
SP - 89
EP - 93
JO - F E B S Letters
JF - F E B S Letters
SN - 0014-5793
IS - 1-2
ER -
ID: 310766240