Potential of ESBL-producing Escherichia coli selection in bovine feces after intramammary administration of first generation cephalosporins using in vitro experiments

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Potential of ESBL-producing Escherichia coli selection in bovine feces after intramammary administration of first generation cephalosporins using in vitro experiments. / Speksnijder, David C.; Hopman, Nonke E.M.; Kusters, Nina E.; Timmerman, Arjen; Swinkels, Jantijn M.; Penterman, Pleun A.A.; Krömker, Volker; Bradley, Andrew J.; Botteldoorn, Nadine; Gehring, Ronette; Zomer, Aldert L.

In: Scientific Reports, Vol. 12, 2022, p. 15083.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Speksnijder, DC, Hopman, NEM, Kusters, NE, Timmerman, A, Swinkels, JM, Penterman, PAA, Krömker, V, Bradley, AJ, Botteldoorn, N, Gehring, R & Zomer, AL 2022, 'Potential of ESBL-producing Escherichia coli selection in bovine feces after intramammary administration of first generation cephalosporins using in vitro experiments', Scientific Reports, vol. 12, pp. 15083. https://doi.org/10.1038/s41598-022-15558-z

APA

Speksnijder, D. C., Hopman, N. E. M., Kusters, N. E., Timmerman, A., Swinkels, J. M., Penterman, P. A. A., Krömker, V., Bradley, A. J., Botteldoorn, N., Gehring, R., & Zomer, A. L. (2022). Potential of ESBL-producing Escherichia coli selection in bovine feces after intramammary administration of first generation cephalosporins using in vitro experiments. Scientific Reports, 12, 15083. https://doi.org/10.1038/s41598-022-15558-z

Vancouver

Speksnijder DC, Hopman NEM, Kusters NE, Timmerman A, Swinkels JM, Penterman PAA et al. Potential of ESBL-producing Escherichia coli selection in bovine feces after intramammary administration of first generation cephalosporins using in vitro experiments. Scientific Reports. 2022;12:15083. https://doi.org/10.1038/s41598-022-15558-z

Author

Speksnijder, David C. ; Hopman, Nonke E.M. ; Kusters, Nina E. ; Timmerman, Arjen ; Swinkels, Jantijn M. ; Penterman, Pleun A.A. ; Krömker, Volker ; Bradley, Andrew J. ; Botteldoorn, Nadine ; Gehring, Ronette ; Zomer, Aldert L. / Potential of ESBL-producing Escherichia coli selection in bovine feces after intramammary administration of first generation cephalosporins using in vitro experiments. In: Scientific Reports. 2022 ; Vol. 12. pp. 15083.

Bibtex

@article{fa648ec0f1d24004b3356ca68a2c8662,

title = "Potential of ESBL-producing Escherichia coli selection in bovine feces after intramammary administration of first generation cephalosporins using in vitro experiments",

abstract = "Selection and spread of Extended Spectrum Beta-Lactamase (ESBL) -producing Enterobacteriaceae within animal production systems and potential spillover to humans is a major concern. Intramammary treatment of dairy cows with first-generation cephalosporins is a common practice and potentially selects for ESBL-producing Enterobacteriaceae, although it is unknown whether this really occurs in the bovine fecal environment. We aimed to study the potential effects of intramammary application of cephapirin (CP) and cefalonium (CL) to select for ESBL-producing Escherichia coli in the intestinal content of treated dairy cows and in manure slurry, using in vitro competition experiments with ESBL and non-ESBL E. coli isolates. No selection of ESBL-producing E. coli was observed at or below concentrations of 0.8 µg/ml and 4.0 µg/ml in bovine feces for CP and CL, respectively, and at or below 8.0 µg/ml and 4.0 µg/ml, respectively, in manure slurry. We calculated that the maximum concentration of CP and CL after intramammary treatment with commercial products will not exceed 0.29 µg/ml in feces and 0.03 µg/ml in manure slurry. Therefore, the results of this study did not find evidence supporting the selection of ESBL-producing E. coli in bovine feces or in manure slurry after intramammary use of commercial CP or CL-containing products.",

author = "Speksnijder, {David C.} and Hopman, {Nonke E.M.} and Kusters, {Nina E.} and Arjen Timmerman and Swinkels, {Jantijn M.} and Penterman, {Pleun A.A.} and Volker Kr{\"o}mker and Bradley, {Andrew J.} and Nadine Botteldoorn and Ronette Gehring and Zomer, {Aldert L.}",

note = "Publisher Copyright: {\textcopyright} 2022. Merck & Co., Inc., Rahway, NJ, USA and its affiliates.",

year = "2022",

doi = "10.1038/s41598-022-15558-z",

language = "English",

volume = "12",

pages = "15083",

journal = "Scientific Reports",

issn = "2045-2322",

publisher = "nature publishing group",

}

RIS

TY - JOUR

T1 - Potential of ESBL-producing Escherichia coli selection in bovine feces after intramammary administration of first generation cephalosporins using in vitro experiments

AU - Speksnijder, David C.

AU - Hopman, Nonke E.M.

AU - Kusters, Nina E.

AU - Timmerman, Arjen

AU - Swinkels, Jantijn M.

AU - Penterman, Pleun A.A.

AU - Krömker, Volker

AU - Bradley, Andrew J.

AU - Botteldoorn, Nadine

AU - Gehring, Ronette

AU - Zomer, Aldert L.

PY - 2022

Y1 - 2022

N2 - Selection and spread of Extended Spectrum Beta-Lactamase (ESBL) -producing Enterobacteriaceae within animal production systems and potential spillover to humans is a major concern. Intramammary treatment of dairy cows with first-generation cephalosporins is a common practice and potentially selects for ESBL-producing Enterobacteriaceae, although it is unknown whether this really occurs in the bovine fecal environment. We aimed to study the potential effects of intramammary application of cephapirin (CP) and cefalonium (CL) to select for ESBL-producing Escherichia coli in the intestinal content of treated dairy cows and in manure slurry, using in vitro competition experiments with ESBL and non-ESBL E. coli isolates. No selection of ESBL-producing E. coli was observed at or below concentrations of 0.8 µg/ml and 4.0 µg/ml in bovine feces for CP and CL, respectively, and at or below 8.0 µg/ml and 4.0 µg/ml, respectively, in manure slurry. We calculated that the maximum concentration of CP and CL after intramammary treatment with commercial products will not exceed 0.29 µg/ml in feces and 0.03 µg/ml in manure slurry. Therefore, the results of this study did not find evidence supporting the selection of ESBL-producing E. coli in bovine feces or in manure slurry after intramammary use of commercial CP or CL-containing products.

AB - Selection and spread of Extended Spectrum Beta-Lactamase (ESBL) -producing Enterobacteriaceae within animal production systems and potential spillover to humans is a major concern. Intramammary treatment of dairy cows with first-generation cephalosporins is a common practice and potentially selects for ESBL-producing Enterobacteriaceae, although it is unknown whether this really occurs in the bovine fecal environment. We aimed to study the potential effects of intramammary application of cephapirin (CP) and cefalonium (CL) to select for ESBL-producing Escherichia coli in the intestinal content of treated dairy cows and in manure slurry, using in vitro competition experiments with ESBL and non-ESBL E. coli isolates. No selection of ESBL-producing E. coli was observed at or below concentrations of 0.8 µg/ml and 4.0 µg/ml in bovine feces for CP and CL, respectively, and at or below 8.0 µg/ml and 4.0 µg/ml, respectively, in manure slurry. We calculated that the maximum concentration of CP and CL after intramammary treatment with commercial products will not exceed 0.29 µg/ml in feces and 0.03 µg/ml in manure slurry. Therefore, the results of this study did not find evidence supporting the selection of ESBL-producing E. coli in bovine feces or in manure slurry after intramammary use of commercial CP or CL-containing products.

U2 - 10.1038/s41598-022-15558-z

DO - 10.1038/s41598-022-15558-z

M3 - Journal article

C2 - 36065056

AN - SCOPUS:85137245444

VL - 12

SP - 15083

JO - Scientific Reports

JF - Scientific Reports

SN - 2045-2322

ER -

ID: 319400661

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