Oxidative damage to guanine nucleosides following combination chemotherapy with 5-fluorouracil and oxaliplatin
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Oxidative damage to guanine nucleosides following combination chemotherapy with 5-fluorouracil and oxaliplatin. / Afzal, Shoaib; Jensen, Søren Astrup; Sørensen, Jens Benn; Henriksen, Trine; Weimann, Allan; Poulsen, Henrik Enghusen.
In: Cancer Chemotherapy and Pharmacology, Vol. 69, 2011, p. 301-307.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Oxidative damage to guanine nucleosides following combination chemotherapy with 5-fluorouracil and oxaliplatin
AU - Afzal, Shoaib
AU - Jensen, Søren Astrup
AU - Sørensen, Jens Benn
AU - Henriksen, Trine
AU - Weimann, Allan
AU - Poulsen, Henrik Enghusen
PY - 2011
Y1 - 2011
N2 - PURPOSE: Recent in vitro and animal studies have suggested that the cytotoxicity of 5-fluorouracil and oxaliplatin is linked to increased formation of reactive oxygen species (ROS). This prospective study was undertaken to examine the generation of oxidative stress, in 106 colorectal cancer patients, by 5-fluorouracil and oxaliplatin combination (FOLFOX) therapy as measured by urinary excretion of 8-oxo-7,8-dihydro-2-deoxyguanosine (8-oxodG) and 8-oxo-7,8-dihydro-guanosine (8-oxoGuo). METHODS: The amounts of 8-oxoGuo and 8-oxodG were measured in 3 spot urine samples from 106 patients by using ultra performance liquid chromatography and tandem mass spectrometry. Furthermore, we collected information on other clinical and demographic variables hypothesized to be associated with oxidative stress. Repeated measures linear mixed models were used to model the relationship between urinary concentrations of 8-oxoGuo and 8-oxodG and the treatment effect and the other variables. RESULTS: The analysis showed that chemotherapy increased the excretion of 8-oxoGuo and 8-oxodG around 15% (P
AB - PURPOSE: Recent in vitro and animal studies have suggested that the cytotoxicity of 5-fluorouracil and oxaliplatin is linked to increased formation of reactive oxygen species (ROS). This prospective study was undertaken to examine the generation of oxidative stress, in 106 colorectal cancer patients, by 5-fluorouracil and oxaliplatin combination (FOLFOX) therapy as measured by urinary excretion of 8-oxo-7,8-dihydro-2-deoxyguanosine (8-oxodG) and 8-oxo-7,8-dihydro-guanosine (8-oxoGuo). METHODS: The amounts of 8-oxoGuo and 8-oxodG were measured in 3 spot urine samples from 106 patients by using ultra performance liquid chromatography and tandem mass spectrometry. Furthermore, we collected information on other clinical and demographic variables hypothesized to be associated with oxidative stress. Repeated measures linear mixed models were used to model the relationship between urinary concentrations of 8-oxoGuo and 8-oxodG and the treatment effect and the other variables. RESULTS: The analysis showed that chemotherapy increased the excretion of 8-oxoGuo and 8-oxodG around 15% (P
U2 - 10.1007/s00280-011-1700-2
DO - 10.1007/s00280-011-1700-2
M3 - Journal article
VL - 69
SP - 301
EP - 307
JO - Cancer Chemotherapy and Pharmacology, Supplement
JF - Cancer Chemotherapy and Pharmacology, Supplement
SN - 0943-9404
ER -
ID: 34090500