Optimization of RNA preservation in granulation tissue in the expanded polytetrafluoroethylene (ePTFE) tube model
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Optimization of RNA preservation in granulation tissue in the expanded polytetrafluoroethylene (ePTFE) tube model. / Danielsen, Patricia L; Koudahl, V; Christensen, B; Olsen, Jørgen.
In: Preparative Biochemistry and Biotechnology, Vol. 39, No. 4, 2009, p. 413-8.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Optimization of RNA preservation in granulation tissue in the expanded polytetrafluoroethylene (ePTFE) tube model
AU - Danielsen, Patricia L
AU - Koudahl, V
AU - Christensen, B
AU - Olsen, Jørgen
PY - 2009
Y1 - 2009
N2 - RNA is easily degraded by RNAses. An efficient and non-hazardous method optimizing RNA preservation and extraction is needed. Our aim was to evaluate RNA preservation methods on murine granulation tissue from subcutaneously implanted ePTFE tubes. ePTFE tubes were placed subcutaneously in mice, removed after ten days and randomized to four RNA preservation groups. Storage in RNAlater at 4 degrees C and snap freezing in methanol/acetone yielded significantly better RNA quality than snap freezing with liquid nitrogen and storage in RNAlater at -80 degrees C. Snap freezing in liquid nitrogen failed to yield intact RNA. We recommend storage in RNAlater at 4 degrees C.
AB - RNA is easily degraded by RNAses. An efficient and non-hazardous method optimizing RNA preservation and extraction is needed. Our aim was to evaluate RNA preservation methods on murine granulation tissue from subcutaneously implanted ePTFE tubes. ePTFE tubes were placed subcutaneously in mice, removed after ten days and randomized to four RNA preservation groups. Storage in RNAlater at 4 degrees C and snap freezing in methanol/acetone yielded significantly better RNA quality than snap freezing with liquid nitrogen and storage in RNAlater at -80 degrees C. Snap freezing in liquid nitrogen failed to yield intact RNA. We recommend storage in RNAlater at 4 degrees C.
U2 - 10.1080/10826060903209695
DO - 10.1080/10826060903209695
M3 - Journal article
C2 - 19739027
VL - 39
SP - 413
EP - 418
JO - Preparative Biochemistry and Biotechnology
JF - Preparative Biochemistry and Biotechnology
SN - 1082-6068
IS - 4
ER -
ID: 14801677