Novel assays to assess the functional capacity of the classical, the alternative and the lectin pathways of the complement system
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Novel assays to assess the functional capacity of the classical, the alternative and the lectin pathways of the complement system. / Palarasah, Y; Nielsen, C; Sprogøe, U; Christensen, M L; Lillevang, S; Madsen, H O; Bygum, A; Koch, C; Skjodt, K; Skjoedt, M-O.
In: Clinical and Experimental Immunology, Vol. 164, No. 3, 06.2011, p. 388-95.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Novel assays to assess the functional capacity of the classical, the alternative and the lectin pathways of the complement system
AU - Palarasah, Y
AU - Nielsen, C
AU - Sprogøe, U
AU - Christensen, M L
AU - Lillevang, S
AU - Madsen, H O
AU - Bygum, A
AU - Koch, C
AU - Skjodt, K
AU - Skjoedt, M-O
N1 - © 2011 The Authors. Clinical and Experimental Immunology © 2011 British Society for Immunology.
PY - 2011/6
Y1 - 2011/6
N2 - Deficiencies in many of the complement proteins and their regulatory molecules have been described and a variety of diseases, such as recurrent infections, systemic lupus erythematosus (SLE) and renal diseases, may be linked to deficiency in the complement system. Screening for complement defects is therefore of great importance. In this study, we present novel improved enzyme-linked immunosorbent assays for the functional assessment of the three individual pathways of the complement system. The method is applicable at high serum concentrations and we demonstrate that it minimizes both false negative as well as false positive results. In particular, for the functional mannose-binding lectin activity it represents an improvement on the existing assays. In this respect, the present assays represent novel improved diagnostic protocols for patients with suspected immunodeficiencies related to the complement system.
AB - Deficiencies in many of the complement proteins and their regulatory molecules have been described and a variety of diseases, such as recurrent infections, systemic lupus erythematosus (SLE) and renal diseases, may be linked to deficiency in the complement system. Screening for complement defects is therefore of great importance. In this study, we present novel improved enzyme-linked immunosorbent assays for the functional assessment of the three individual pathways of the complement system. The method is applicable at high serum concentrations and we demonstrate that it minimizes both false negative as well as false positive results. In particular, for the functional mannose-binding lectin activity it represents an improvement on the existing assays. In this respect, the present assays represent novel improved diagnostic protocols for patients with suspected immunodeficiencies related to the complement system.
KW - Adult
KW - Aged
KW - Complement Pathway, Alternative
KW - Complement Pathway, Classical
KW - Complement Pathway, Mannose-Binding Lectin
KW - Enzyme-Linked Immunosorbent Assay
KW - False Negative Reactions
KW - False Positive Reactions
KW - Female
KW - Humans
KW - Infection
KW - Kidney Diseases, Cystic
KW - Lupus Erythematosus, Systemic
KW - Male
KW - Middle Aged
KW - Comparative Study
KW - Journal Article
KW - Research Support, Non-U.S. Gov't
U2 - 10.1111/j.1365-2249.2011.04322.x
DO - 10.1111/j.1365-2249.2011.04322.x
M3 - Journal article
C2 - 21401574
VL - 164
SP - 388
EP - 395
JO - Clinical and Experimental Immunology
JF - Clinical and Experimental Immunology
SN - 0009-9104
IS - 3
ER -
ID: 172399523