Non-invasive method for sampling and extraction of mouse DNA for PCR
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Non-invasive method for sampling and extraction of mouse DNA for PCR. / Meldgaard, M; Bollen, P J A; Finsen, B.
In: Laboratory Animals, Vol. 38, No. 4, 2004, p. 413-417.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Non-invasive method for sampling and extraction of mouse DNA for PCR
AU - Meldgaard, M
AU - Bollen, P J A
AU - Finsen, B
PY - 2004
Y1 - 2004
N2 - We adapted a non-invasive, fast, reliable and inexpensive procedure for the sampling and extraction of deoxyribonucleic acid (DNA) for genetic testing of mice. The procedure is based on a simple DNA extraction procedure used in the forensic genetic testing of humans. It involves mouth swabbing of the inner cheek using a cotton stick, followed by alkaline lysis of the harvested buccal epithelial cells. This procedure allows for repeated sampling and genetic testing of the individual mouse, and it is faster, simpler and, in our hands, more reliable than the currently used routine procedures for the sampling and extraction of mouse DNA. Current procedures all involve biopsy of a piece of the tail, ear or toe, followed by lengthy procedures to release and isolate the DNA.
AB - We adapted a non-invasive, fast, reliable and inexpensive procedure for the sampling and extraction of deoxyribonucleic acid (DNA) for genetic testing of mice. The procedure is based on a simple DNA extraction procedure used in the forensic genetic testing of humans. It involves mouth swabbing of the inner cheek using a cotton stick, followed by alkaline lysis of the harvested buccal epithelial cells. This procedure allows for repeated sampling and genetic testing of the individual mouse, and it is faster, simpler and, in our hands, more reliable than the currently used routine procedures for the sampling and extraction of mouse DNA. Current procedures all involve biopsy of a piece of the tail, ear or toe, followed by lengthy procedures to release and isolate the DNA.
KW - Animals
KW - Cheek/physiology
KW - DNA/chemistry
KW - Epithelial Cells/chemistry
KW - Mice/genetics
KW - Polymerase Chain Reaction/methods
KW - Saliva/chemistry
KW - Specimen Handling/methods
U2 - 10.1258/0023677041958981
DO - 10.1258/0023677041958981
M3 - Journal article
C2 - 15479556
VL - 38
SP - 413
EP - 417
JO - Laboratory Animals
JF - Laboratory Animals
SN - 0023-6772
IS - 4
ER -
ID: 324128689