Noninvasive measurement of reepithelialization and microvascularity of suction-blister wounds with benchmarking to histology
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Noninvasive measurement of reepithelialization and microvascularity of suction-blister wounds with benchmarking to histology. / Larsen, Heidi F.; Ahlström, Malin G.; Gjerdrum, Lise M.R.; Mogensen, Mette; Ghathian, Khaled; Calum, Henrik; Sørensen, Anne L.; Forman, Julie L.; Vandeven, Mark; Holerca, Marian N.; Du-Thumm, Laurence; Jorgensen, Lars N.; Ågren, Magnus S.
In: Wound Repair and Regeneration, Vol. 25, No. 6, 11.2017, p. 984-993.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Noninvasive measurement of reepithelialization and microvascularity of suction-blister wounds with benchmarking to histology
AU - Larsen, Heidi F.
AU - Ahlström, Malin G.
AU - Gjerdrum, Lise M.R.
AU - Mogensen, Mette
AU - Ghathian, Khaled
AU - Calum, Henrik
AU - Sørensen, Anne L.
AU - Forman, Julie L.
AU - Vandeven, Mark
AU - Holerca, Marian N.
AU - Du-Thumm, Laurence
AU - Jorgensen, Lars N.
AU - Ågren, Magnus S.
PY - 2017/11
Y1 - 2017/11
N2 - We explored use of the suction-blister wound model in the assessment of not only epidermal regeneration but also pain, the microvascular response and bacteriology. The effects of topical zinc sulfate were studied to articulate the methodologies in this double-blind trial. One epidermal suction blister (10 mm) was induced on each buttock in 30 healthy volunteers (15 females:15 males) and deroofed on day 0. The wounds were randomized to daily treatment with 1.4% zinc sulfate shower gel (n = 20), placebo (n = 20) or control (n = 20). Digital photography coupled with planimetry, transepidermal water loss (TEWL) measurement and optical coherence tomography (OCT) was benchmarked to the gold standard of histology of 60 full-thickness wound biopsies on day 4. Pain increased after application of the shower gels. Microvessel density, determined from OCT images, increased from day 0 to day 2 in the three groups but increased more with the placebo than with the zinc shower gel (p = 0.003) or the control treatment (p = 0.002) and correlated (rS = 0.313, p = 0.015) with the inflammatory response on day 4, as determined by histology. Coagulase-negative staphylococci were more common in wounds compared with skin (p = 0.002) and was reduced (p = 0.030) with zinc sulfate treatment. Planimetric analysis of digital wound images was not biased (p = 0.234) compared with histology, and TEWL measurements showed no correlation (rS = 0.052, p = 0.691) with epithelialization. Neoepidermal formation, determined by histology, did not differ (p = 0.290) among the groups. Zinc sulfate reduced (p = 0.031) the release of lactate dehydrogenase from cultured gel-treated keratinocytes isolated from the blister roofs. Therefore, combination of the standardized suction-blister wound model with noninvasive planimetry and OCT is a useful tool for assessing wound therapies. Zinc sulfate transiently dampened inflammation and reduced bacterial growth.
AB - We explored use of the suction-blister wound model in the assessment of not only epidermal regeneration but also pain, the microvascular response and bacteriology. The effects of topical zinc sulfate were studied to articulate the methodologies in this double-blind trial. One epidermal suction blister (10 mm) was induced on each buttock in 30 healthy volunteers (15 females:15 males) and deroofed on day 0. The wounds were randomized to daily treatment with 1.4% zinc sulfate shower gel (n = 20), placebo (n = 20) or control (n = 20). Digital photography coupled with planimetry, transepidermal water loss (TEWL) measurement and optical coherence tomography (OCT) was benchmarked to the gold standard of histology of 60 full-thickness wound biopsies on day 4. Pain increased after application of the shower gels. Microvessel density, determined from OCT images, increased from day 0 to day 2 in the three groups but increased more with the placebo than with the zinc shower gel (p = 0.003) or the control treatment (p = 0.002) and correlated (rS = 0.313, p = 0.015) with the inflammatory response on day 4, as determined by histology. Coagulase-negative staphylococci were more common in wounds compared with skin (p = 0.002) and was reduced (p = 0.030) with zinc sulfate treatment. Planimetric analysis of digital wound images was not biased (p = 0.234) compared with histology, and TEWL measurements showed no correlation (rS = 0.052, p = 0.691) with epithelialization. Neoepidermal formation, determined by histology, did not differ (p = 0.290) among the groups. Zinc sulfate reduced (p = 0.031) the release of lactate dehydrogenase from cultured gel-treated keratinocytes isolated from the blister roofs. Therefore, combination of the standardized suction-blister wound model with noninvasive planimetry and OCT is a useful tool for assessing wound therapies. Zinc sulfate transiently dampened inflammation and reduced bacterial growth.
U2 - 10.1111/wrr.12605
DO - 10.1111/wrr.12605
M3 - Journal article
C2 - 29316016
AN - SCOPUS:85042064156
VL - 25
SP - 984
EP - 993
JO - Wound Repair and Regeneration
JF - Wound Repair and Regeneration
SN - 1067-1927
IS - 6
ER -
ID: 196438853