No junctional communication between epithelial cells in hydra
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No junctional communication between epithelial cells in hydra. / de Laat, S W; Tertoolen, L G; Grimmelikhuijzen, C J.
In: Nature, Vol. 288, No. 5792, 25.12.1980, p. 711-3.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - No junctional communication between epithelial cells in hydra
AU - de Laat, S W
AU - Tertoolen, L G
AU - Grimmelikhuijzen, C J
PY - 1980/12/25
Y1 - 1980/12/25
N2 - Diffusion gradients of morphogens have been inferred as a basis for the control of morphogenesis in hydra, and morphogenetic substances have been found which, on the basis of their molecular weight (MW), should be able to pass gap junctions. There have been several reports of the presence of gap junctions between epithelial cells of hydra. However, until now, there has been no report published on whether these junctions enable the epithelial cells to exchange molecules of small molecular weight, as has been described in other organisms. Therefore we decided to investigate the communicative properties of the junctional membranes by electrophysiological methods and by intracellular-dye iontophoresis. We report here that no electrotonic coupling is detectable between epithelial cells of Hydra attenuata in: (1) intact animals, (2) head-regenerating animals, (3) cell re-aggregates, and (4) hydra that have become nervefree. Furthermore we show that epithelial cells are unable to exchange low-molecular weight fluorescent dyes.
AB - Diffusion gradients of morphogens have been inferred as a basis for the control of morphogenesis in hydra, and morphogenetic substances have been found which, on the basis of their molecular weight (MW), should be able to pass gap junctions. There have been several reports of the presence of gap junctions between epithelial cells of hydra. However, until now, there has been no report published on whether these junctions enable the epithelial cells to exchange molecules of small molecular weight, as has been described in other organisms. Therefore we decided to investigate the communicative properties of the junctional membranes by electrophysiological methods and by intracellular-dye iontophoresis. We report here that no electrotonic coupling is detectable between epithelial cells of Hydra attenuata in: (1) intact animals, (2) head-regenerating animals, (3) cell re-aggregates, and (4) hydra that have become nervefree. Furthermore we show that epithelial cells are unable to exchange low-molecular weight fluorescent dyes.
KW - Animals
KW - Cell Communication
KW - Ectoderm
KW - Electrophysiology
KW - Endoderm
KW - Fluorescent Dyes
KW - Hydra
KW - Intercellular Junctions
KW - Membrane Potentials
M3 - Journal article
C2 - 7453801
VL - 288
SP - 711
EP - 713
JO - Nature
JF - Nature
SN - 0028-0836
IS - 5792
ER -
ID: 33514936