Next-generation museum genomics: Phylogenetic relationships among palpimanoid spiders using sequence capture techniques (Araneae: Palpimanoidea)
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Next-generation museum genomics : Phylogenetic relationships among palpimanoid spiders using sequence capture techniques (Araneae: Palpimanoidea). / Wood, Hannah Maria; González, Vanessa; Lloyd, Michael; Coddington, Jonathan A.; Scharff, Nikolaj.
In: Molecular Phylogenetics and Evolution, Vol. 127, 2018, p. 907-918.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Next-generation museum genomics
T2 - Phylogenetic relationships among palpimanoid spiders using sequence capture techniques (Araneae: Palpimanoidea)
AU - Wood, Hannah Maria
AU - González, Vanessa
AU - Lloyd, Michael
AU - Coddington, Jonathan A.
AU - Scharff, Nikolaj
PY - 2018
Y1 - 2018
N2 - Historical museum specimens are invaluable for morphological and taxonomic research, but typically the DNA is degraded making traditional sequencing techniques difficult to impossible for many specimens. Recent advances in Next-Generation Sequencing, specifically target capture, makes use of short fragment sizes typical of degraded DNA, opening up the possibilities for gathering genomic data from museum specimens. This study uses museum specimens and recent target capture sequencing techniques to sequence both Ultra-Conserved Elements (UCE) and exonic regions for lineages that span the modern spiders, Araneomorphae, with a focus on Palpimanoidea. While many previous studies have used target capture techniques on dried museum specimens (for example,skins, pinned insects), this study includes specimens that were collected over the last two decades and stored in 70% ethanol at room temperature. Our findings support the utility of target capture methods for examining deep relationships within Araneomorphae: sequences from both UCE and exonic loci were important for resolving relationships; a monophyletic Palpimanoidea was recovered in many analyses and there was strong support for family and generic-level palpimanoid relationships. Ancestral character state reconstructions reveal that the highly modified carapace observed in mecysmaucheniids and archaeids has evolved independently.
AB - Historical museum specimens are invaluable for morphological and taxonomic research, but typically the DNA is degraded making traditional sequencing techniques difficult to impossible for many specimens. Recent advances in Next-Generation Sequencing, specifically target capture, makes use of short fragment sizes typical of degraded DNA, opening up the possibilities for gathering genomic data from museum specimens. This study uses museum specimens and recent target capture sequencing techniques to sequence both Ultra-Conserved Elements (UCE) and exonic regions for lineages that span the modern spiders, Araneomorphae, with a focus on Palpimanoidea. While many previous studies have used target capture techniques on dried museum specimens (for example,skins, pinned insects), this study includes specimens that were collected over the last two decades and stored in 70% ethanol at room temperature. Our findings support the utility of target capture methods for examining deep relationships within Araneomorphae: sequences from both UCE and exonic loci were important for resolving relationships; a monophyletic Palpimanoidea was recovered in many analyses and there was strong support for family and generic-level palpimanoid relationships. Ancestral character state reconstructions reveal that the highly modified carapace observed in mecysmaucheniids and archaeids has evolved independently.
U2 - 10.1016/j.ympev.2018.06.038
DO - 10.1016/j.ympev.2018.06.038
M3 - Journal article
C2 - 29966686
VL - 127
SP - 907
EP - 918
JO - Molecular Phylogenetics and Evolution
JF - Molecular Phylogenetics and Evolution
SN - 1055-7903
ER -
ID: 200181173