Mouse models of acute and chronic hepacivirus infection

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Mouse models of acute and chronic hepacivirus infection. / Billerbeck, Eva; Wolfisberg, Raphael; Fahnøe, Ulrik; Xiao, Jing W.; Quirk, Corrine; Luna, Joseph M; Cullen, John M; Hartlage, Alex S.; Chiriboga, Luis; Ghoshal, Kalpana; Lipkin, W Ian; Bukh, Jens; Scheel, Troels K.H.; Kapoor, Amit; Rice, Charles M.

In: Science, Vol. 357, No. 6347, 2017, p. 204-208.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Billerbeck, E, Wolfisberg, R, Fahnøe, U, Xiao, JW, Quirk, C, Luna, JM, Cullen, JM, Hartlage, AS, Chiriboga, L, Ghoshal, K, Lipkin, WI, Bukh, J, Scheel, TKH, Kapoor, A & Rice, CM 2017, 'Mouse models of acute and chronic hepacivirus infection', Science, vol. 357, no. 6347, pp. 204-208. https://doi.org/10.1126/science.aal1962

APA

Billerbeck, E., Wolfisberg, R., Fahnøe, U., Xiao, J. W., Quirk, C., Luna, J. M., Cullen, J. M., Hartlage, A. S., Chiriboga, L., Ghoshal, K., Lipkin, W. I., Bukh, J., Scheel, T. K. H., Kapoor, A., & Rice, C. M. (2017). Mouse models of acute and chronic hepacivirus infection. Science, 357(6347), 204-208. https://doi.org/10.1126/science.aal1962

Vancouver

Billerbeck E, Wolfisberg R, Fahnøe U, Xiao JW, Quirk C, Luna JM et al. Mouse models of acute and chronic hepacivirus infection. Science. 2017;357(6347):204-208. https://doi.org/10.1126/science.aal1962

Author

Billerbeck, Eva ; Wolfisberg, Raphael ; Fahnøe, Ulrik ; Xiao, Jing W. ; Quirk, Corrine ; Luna, Joseph M ; Cullen, John M ; Hartlage, Alex S. ; Chiriboga, Luis ; Ghoshal, Kalpana ; Lipkin, W Ian ; Bukh, Jens ; Scheel, Troels K.H. ; Kapoor, Amit ; Rice, Charles M. / Mouse models of acute and chronic hepacivirus infection. In: Science. 2017 ; Vol. 357, No. 6347. pp. 204-208.

Bibtex

@article{ca112ad217a04486ba6f52e34206182f,
title = "Mouse models of acute and chronic hepacivirus infection",
abstract = "An estimated 71 million people worldwide are infected with hepatitis C virus (HCV). The lack of small-animal models has impeded studies of antiviral immune mechanisms. Here we show that an HCV-related hepacivirus discovered in Norway rats can establish high-titer hepatotropic infections in laboratory mice with immunological features resembling those seen in human viral hepatitis. Whereas immune-compromised mice developed persistent infection, immune-competent mice cleared the virus within 3 to 5 weeks. Acute clearance was T cell dependent and associated with liver injury. Transient depletion of CD4+ T cells before infection resulted in chronic infection, characterized by high levels of intrahepatic regulatory T cells and expression of inhibitory molecules on intrahepatic CD8+ T cells. Natural killer cells controlled early infection but were not essential for viral clearance. This model may provide mechanistic insights into hepatic antiviral immunity, a prerequisite for the development of HCV vaccines.",
author = "Eva Billerbeck and Raphael Wolfisberg and Ulrik Fahn{\o}e and Xiao, {Jing W.} and Corrine Quirk and Luna, {Joseph M} and Cullen, {John M} and Hartlage, {Alex S.} and Luis Chiriboga and Kalpana Ghoshal and Lipkin, {W Ian} and Jens Bukh and Scheel, {Troels K.H.} and Amit Kapoor and Rice, {Charles M}",
year = "2017",
doi = "10.1126/science.aal1962",
language = "English",
volume = "357",
pages = "204--208",
journal = "Science",
issn = "0036-8075",
publisher = "American Association for the Advancement of Science",
number = "6347",

}

RIS

TY - JOUR

T1 - Mouse models of acute and chronic hepacivirus infection

AU - Billerbeck, Eva

AU - Wolfisberg, Raphael

AU - Fahnøe, Ulrik

AU - Xiao, Jing W.

AU - Quirk, Corrine

AU - Luna, Joseph M

AU - Cullen, John M

AU - Hartlage, Alex S.

AU - Chiriboga, Luis

AU - Ghoshal, Kalpana

AU - Lipkin, W Ian

AU - Bukh, Jens

AU - Scheel, Troels K.H.

AU - Kapoor, Amit

AU - Rice, Charles M

PY - 2017

Y1 - 2017

N2 - An estimated 71 million people worldwide are infected with hepatitis C virus (HCV). The lack of small-animal models has impeded studies of antiviral immune mechanisms. Here we show that an HCV-related hepacivirus discovered in Norway rats can establish high-titer hepatotropic infections in laboratory mice with immunological features resembling those seen in human viral hepatitis. Whereas immune-compromised mice developed persistent infection, immune-competent mice cleared the virus within 3 to 5 weeks. Acute clearance was T cell dependent and associated with liver injury. Transient depletion of CD4+ T cells before infection resulted in chronic infection, characterized by high levels of intrahepatic regulatory T cells and expression of inhibitory molecules on intrahepatic CD8+ T cells. Natural killer cells controlled early infection but were not essential for viral clearance. This model may provide mechanistic insights into hepatic antiviral immunity, a prerequisite for the development of HCV vaccines.

AB - An estimated 71 million people worldwide are infected with hepatitis C virus (HCV). The lack of small-animal models has impeded studies of antiviral immune mechanisms. Here we show that an HCV-related hepacivirus discovered in Norway rats can establish high-titer hepatotropic infections in laboratory mice with immunological features resembling those seen in human viral hepatitis. Whereas immune-compromised mice developed persistent infection, immune-competent mice cleared the virus within 3 to 5 weeks. Acute clearance was T cell dependent and associated with liver injury. Transient depletion of CD4+ T cells before infection resulted in chronic infection, characterized by high levels of intrahepatic regulatory T cells and expression of inhibitory molecules on intrahepatic CD8+ T cells. Natural killer cells controlled early infection but were not essential for viral clearance. This model may provide mechanistic insights into hepatic antiviral immunity, a prerequisite for the development of HCV vaccines.

U2 - 10.1126/science.aal1962

DO - 10.1126/science.aal1962

M3 - Journal article

C2 - 28706073

AN - SCOPUS:85025478531

VL - 357

SP - 204

EP - 208

JO - Science

JF - Science

SN - 0036-8075

IS - 6347

ER -

ID: 182122953