Morphology of Entomophthora muscae protoplasts grown in vitro
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Morphology of Entomophthora muscae protoplasts grown in vitro. / Latgé, J. P.; Eilenberg, J.; Beauvais, A.; Prevost, M. C.
In: Protoplasma, Vol. 146, No. 2-3, 01.06.1988, p. 166-173.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Morphology of Entomophthora muscae protoplasts grown in vitro
AU - Latgé, J. P.
AU - Eilenberg, J.
AU - Beauvais, A.
AU - Prevost, M. C.
PY - 1988/6/1
Y1 - 1988/6/1
N2 - Entomophthora muscae (C.) Fres. can be grown in vitro as protoplasts. Light and electron microscopical studies of the in vitro developed protoplasts have demonstrated the absence of an organized wall over the protoplasmic Con A-positive membrane at all stages of growth. The cytological organization is typical of the Entomophthorales with condensed chromatin in the interphase nuclei and small eccentric metaphase spindles. Long strands of endoplasmic reticulum, microubules and vesicles surrounding the plasmalemma may be involved in maintaining the precise shape of E. muscae protoplast. Starvation of the fungus induces the formation of hyphal bodies after deposition of Con A- and WGA-positive wall material at the plasmalemma surface.
AB - Entomophthora muscae (C.) Fres. can be grown in vitro as protoplasts. Light and electron microscopical studies of the in vitro developed protoplasts have demonstrated the absence of an organized wall over the protoplasmic Con A-positive membrane at all stages of growth. The cytological organization is typical of the Entomophthorales with condensed chromatin in the interphase nuclei and small eccentric metaphase spindles. Long strands of endoplasmic reticulum, microubules and vesicles surrounding the plasmalemma may be involved in maintaining the precise shape of E. muscae protoplast. Starvation of the fungus induces the formation of hyphal bodies after deposition of Con A- and WGA-positive wall material at the plasmalemma surface.
KW - Cytochemistry
KW - Entomophthora muscae
KW - Entomophthorales
KW - in vitro growth
KW - Protoplast
KW - Ultrastructure
UR - http://www.scopus.com/inward/record.url?scp=0008584401&partnerID=8YFLogxK
U2 - 10.1007/BF01405926
DO - 10.1007/BF01405926
M3 - Journal article
AN - SCOPUS:0008584401
VL - 146
SP - 166
EP - 173
JO - Protoplasma
JF - Protoplasma
SN - 0033-183X
IS - 2-3
ER -
ID: 200827641