Microscale purification of proteins by line immunoelectrophoresis: application of the technique in protein biogenesis studies
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Microscale purification of proteins by line immunoelectrophoresis: application of the technique in protein biogenesis studies. / Danielsen, E M; Cowell, G M.
In: Journal of Proteomics, Vol. 8, No. 1, 1983, p. 41-7.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Microscale purification of proteins by line immunoelectrophoresis: application of the technique in protein biogenesis studies
AU - Danielsen, E M
AU - Cowell, G M
N1 - Keywords: Animals; Immunoelectrophoresis; Intestine, Small; Microvilli; Protein Biosynthesis; Proteins; Swine
PY - 1983
Y1 - 1983
N2 - A small-scale version of line immunoelectrophoresis in combination with immunoprecipitate excision is described as a rapid and convenient technique to purify proteins on a micro scale in biogenesis studies. In the purification and to result in a higher state of purity than an isolation procedure using protein A-Sepharose. Since the method furthermore allows a simultaneous purification of several different protein antigens from the same sample, it may be of interest as an alternative method to other procedures in the purification of proteins on a micro scale.
AB - A small-scale version of line immunoelectrophoresis in combination with immunoprecipitate excision is described as a rapid and convenient technique to purify proteins on a micro scale in biogenesis studies. In the purification and to result in a higher state of purity than an isolation procedure using protein A-Sepharose. Since the method furthermore allows a simultaneous purification of several different protein antigens from the same sample, it may be of interest as an alternative method to other procedures in the purification of proteins on a micro scale.
M3 - Journal article
C2 - 6630867
VL - 8
SP - 41
EP - 47
JO - Journal of Proteomics
JF - Journal of Proteomics
SN - 1874-3919
IS - 1
ER -
ID: 9881450