Metabolism and inflammatory mediators in the peritendinous space measured by microdialysis during intermittent isometric exercise in humans
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Metabolism and inflammatory mediators in the peritendinous space measured by microdialysis during intermittent isometric exercise in humans. / Langberg, Henning; Skovgaard, D; Karamouzis, M; Bülow, J; Kjaer, M.
In: Journal of Physiology, Vol. 515 ( Pt 3), 1999, p. 919-27.Research output: Contribution to journal › Journal article › Research › peer-review
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T1 - Metabolism and inflammatory mediators in the peritendinous space measured by microdialysis during intermittent isometric exercise in humans
AU - Langberg, Henning
AU - Skovgaard, D
AU - Karamouzis, M
AU - Bülow, J
AU - Kjaer, M
PY - 1999
Y1 - 1999
N2 - 1. The metabolic processes that occur around the tendon during mechanical loading and exercise are undescribed in man. These processes are important for understanding the development of overuse inflammation and injury. 2. A microdialysis technique was used to determine interstitial concentrations of glycerol, glucose, lactate, prostaglandin E2 (PGE2) and thromboxane B2 (TXB2) as well as to calculate tissue substrate balance in the peritendinous region of the human Achilles tendon. Recovery of 48-62 % (range) at rest and 70-77 % during exercise were obtained for glycerol, glucose and PGE2. 3. Six young healthy humans were studied at rest, during 30 min of intermittent static plantar flexion of the ankle at a workload corresponding to individual body weight, and during 60 min of recovery. Microdialysis was performed in both legs with simultaneous determination of blood flow by 133Xe washout in the same area, and blood sampling from the radial artery. 4. With exercise, the net release of lactate as well as of glycerol from the peritendinous space of the Achilles tendon increased 2-fold (P <0.05). Furthermore a 100 % increase in interstitial concentration of PGE2 and TXB2 was found, but it was only significant for TXB2(P <0.05). As peritendinous blood flow increased 2- to 3-fold during intermittent static contractions, this indicates also that the output of these substances from the tissue increased during exercise. 5. This study indicates that both lipid and carbohydrate metabolism as well as inflammatory activity is accelerated in the peritendinous region of the human Achilles tendon with dynamic loading.
AB - 1. The metabolic processes that occur around the tendon during mechanical loading and exercise are undescribed in man. These processes are important for understanding the development of overuse inflammation and injury. 2. A microdialysis technique was used to determine interstitial concentrations of glycerol, glucose, lactate, prostaglandin E2 (PGE2) and thromboxane B2 (TXB2) as well as to calculate tissue substrate balance in the peritendinous region of the human Achilles tendon. Recovery of 48-62 % (range) at rest and 70-77 % during exercise were obtained for glycerol, glucose and PGE2. 3. Six young healthy humans were studied at rest, during 30 min of intermittent static plantar flexion of the ankle at a workload corresponding to individual body weight, and during 60 min of recovery. Microdialysis was performed in both legs with simultaneous determination of blood flow by 133Xe washout in the same area, and blood sampling from the radial artery. 4. With exercise, the net release of lactate as well as of glycerol from the peritendinous space of the Achilles tendon increased 2-fold (P <0.05). Furthermore a 100 % increase in interstitial concentration of PGE2 and TXB2 was found, but it was only significant for TXB2(P <0.05). As peritendinous blood flow increased 2- to 3-fold during intermittent static contractions, this indicates also that the output of these substances from the tissue increased during exercise. 5. This study indicates that both lipid and carbohydrate metabolism as well as inflammatory activity is accelerated in the peritendinous region of the human Achilles tendon with dynamic loading.
KW - Achilles Tendon
KW - Adult
KW - Ankle Joint
KW - Body Weight
KW - Dinoprostone
KW - Exercise
KW - Female
KW - Glucose
KW - Glycerol
KW - Humans
KW - Lactates
KW - Male
KW - Microdialysis
KW - Regional Blood Flow
KW - Thromboxane B2
KW - Xenon Radioisotopes
M3 - Journal article
C2 - 10066916
VL - 515 ( Pt 3)
SP - 919
EP - 927
JO - The Journal of Physiology
JF - The Journal of Physiology
SN - 0022-3751
ER -
ID: 38368740