Measuring rosetting inhibition in Plasmodium falciparum parasites using a flow cytometry-based assay
Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review
Rosetting is the ability of Plasmodium falciparum-infected erythrocytes (IEs) to bind to host receptors on the surface of uninfected erythrocytes (uE) leading to the formation of a cluster of cells with a central IE surrounded by uE. It is a hallmark event during the pathogenesis of P. falciparum malaria, the most severe species causing malaria, which affects mostly young children in Africa. There are no current treatments effectively targeting and disrupting parasite rosette formation. Here, we detail a high-throughput, flow cytometry based assay that allows testing and identification of potential rosetting-inhibitory compounds that could be used in combination with anti-plasmodial drugs to reduce malaria morbidity and mortality.
Original language | English |
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Title of host publication | Malaria Immunology : Targeting the Surface of Infected Erythrocytes |
Number of pages | 11 |
Volume | 2470 |
Publication date | 2022 |
Pages | 493-503 |
ISBN (Print) | 978-1-0716-2188-2 |
ISBN (Electronic) | 978-1-0716-2189-9 |
DOIs | |
Publication status | Published - 2022 |
Series | Methods in molecular biology (Clifton, N.J.) |
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ISSN | 1064-3745 |
Bibliographical note
© 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.
- Animals, Child, Child, Preschool, Erythrocytes/metabolism, Flow Cytometry, Humans, Malaria, Falciparum/parasitology, Parasites, Plasmodium falciparum/physiology, Rosette Formation
Research areas
ID: 317099505