Measurement of bacterial gene expression in vivo by laser capture microdissection and quantitative real-time RT-PCR
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Measurement of bacterial gene expression in vivo by laser capture microdissection and quantitative real-time RT-PCR. / Schou, Kirstine Klitgaard; Jensen, Tim Kåre; Angen, Øystein; Boye, Mette.
In: Journal of Microbiological Methods, 2007.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Measurement of bacterial gene expression in vivo by laser capture microdissection and quantitative real-time RT-PCR
AU - Schou, Kirstine Klitgaard
AU - Jensen, Tim Kåre
AU - Angen, Øystein
AU - Boye, Mette
PY - 2007
Y1 - 2007
N2 - Due to the relative small number of bacterial pathogens present in an infected host, exploration of pathogen gene expression in vivo is challenging. This study reports the development of a protocol for quantifying bacterial gene expression in vivo in Actinobacillus pleuropneumoniae using laser capture microdissection and real-time quantitative RT-PCR.
AB - Due to the relative small number of bacterial pathogens present in an infected host, exploration of pathogen gene expression in vivo is challenging. This study reports the development of a protocol for quantifying bacterial gene expression in vivo in Actinobacillus pleuropneumoniae using laser capture microdissection and real-time quantitative RT-PCR.
U2 - 10.1016/j.mimet.2006.12.003
DO - 10.1016/j.mimet.2006.12.003
M3 - Journal article
C2 - 17250913
JO - Journal of Microbiological Methods
JF - Journal of Microbiological Methods
SN - 0167-7012
ER -
ID: 339891875