A set of 25 strains belonging to clade V of Mannheimia mainly isolated from cattle was investigated and is proposed to represent Mannheimia indoligenes sp. nov. The species can be separated from the other validly published species of the genus by pheno-and genotype. Only indole separates M. indoligenes and Mannheimia varigena while two to seven characters separate M. indoligenes from other species of Mannheimia. Thirteen strains belonging to biogroups 6, 7, 8C, 9, 10, 12 and UG5 formed a monophyletic group based on 16S rRNA gene sequence comparisons with 98–100% similarity. Eight of these strains were further included in the whole genome comparison. Digital DNA–DNA hybridization showed that the similarities between the suggested type strain M14.4^T and the other strains of M. indoligenes were 62.9% or higher. The average nucleotide identity was 95.5% or higher between M14.4^T and the other strains of the species. The rpoB gene sequence similarity was 95–100% within M. indoligenes. MALDI-TOF allowed a clear separation from other Mannheimia species further supporting classification as a novel species and making it the diagnostic identification tool of choice for M. indoligenes. The type strain is M14.4^T (=CCUG 77347^T=DSM 116804^T) isolated from a cattle tongue in Scotland.