Long-term feeder-free culture of human pancreatic progenitors on fibronectin or matrix-free polymer potentiates β cell differentiation

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Long-term feeder-free culture of human pancreatic progenitors on fibronectin or matrix-free polymer potentiates β cell differentiation. / Nakamura, Akiko; Wong, Yan Fung; Venturato, Andrea; Michaut, Magali; Venkateswaran, Seshasailam; Santra, Mithun; Gonçalves, Carla; Larsen, Michael; Leuschner, Marit; Kim, Yung Hae; Brickman, Joshua; Bradley, Mark; Grapin-Botton, Anne.

In: Stem Cell Reports, Vol. 17, No. 5, 2022, p. 1215-1228.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Nakamura, A, Wong, YF, Venturato, A, Michaut, M, Venkateswaran, S, Santra, M, Gonçalves, C, Larsen, M, Leuschner, M, Kim, YH, Brickman, J, Bradley, M & Grapin-Botton, A 2022, 'Long-term feeder-free culture of human pancreatic progenitors on fibronectin or matrix-free polymer potentiates β cell differentiation', Stem Cell Reports, vol. 17, no. 5, pp. 1215-1228. https://doi.org/10.1016/j.stemcr.2022.03.013

APA

Nakamura, A., Wong, Y. F., Venturato, A., Michaut, M., Venkateswaran, S., Santra, M., Gonçalves, C., Larsen, M., Leuschner, M., Kim, Y. H., Brickman, J., Bradley, M., & Grapin-Botton, A. (2022). Long-term feeder-free culture of human pancreatic progenitors on fibronectin or matrix-free polymer potentiates β cell differentiation. Stem Cell Reports, 17(5), 1215-1228. https://doi.org/10.1016/j.stemcr.2022.03.013

Vancouver

Nakamura A, Wong YF, Venturato A, Michaut M, Venkateswaran S, Santra M et al. Long-term feeder-free culture of human pancreatic progenitors on fibronectin or matrix-free polymer potentiates β cell differentiation. Stem Cell Reports. 2022;17(5):1215-1228. https://doi.org/10.1016/j.stemcr.2022.03.013

Author

Nakamura, Akiko ; Wong, Yan Fung ; Venturato, Andrea ; Michaut, Magali ; Venkateswaran, Seshasailam ; Santra, Mithun ; Gonçalves, Carla ; Larsen, Michael ; Leuschner, Marit ; Kim, Yung Hae ; Brickman, Joshua ; Bradley, Mark ; Grapin-Botton, Anne. / Long-term feeder-free culture of human pancreatic progenitors on fibronectin or matrix-free polymer potentiates β cell differentiation. In: Stem Cell Reports. 2022 ; Vol. 17, No. 5. pp. 1215-1228.

Bibtex

@article{168090207f86476fbfcf8da12009de40,
title = "Long-term feeder-free culture of human pancreatic progenitors on fibronectin or matrix-free polymer potentiates β cell differentiation",
abstract = "With the aim of producing β cells for replacement therapies to treat diabetes, several protocols have been developed to differentiate human pluripotent stem cells to β cells via pancreatic progenitors. While in vivo pancreatic progenitors expand throughout development, the in vitro protocols have been designed to make these cells progress as fast as possible to β cells. Here, we report on a protocol enabling a long-term expansion of human pancreatic progenitors in a defined medium on fibronectin, in the absence of feeder layers. Moreover, through a screening of a polymer library we identify a polymer that can replace fibronectin. Our experiments, comparing expanded progenitors to directly differentiated progenitors, show that the expanded progenitors differentiate more efficiently into glucose-responsive β cells and produce fewer glucagon-expressing cells. The ability to expand progenitors under defined conditions and cryopreserve them will provide flexibility in research and therapeutic production.",
keywords = "beta cells, differentiation, endocrine cells, human, long-term culture, pancreas, pancreatic progenitors, screening, transcriptome",
author = "Akiko Nakamura and Wong, {Yan Fung} and Andrea Venturato and Magali Michaut and Seshasailam Venkateswaran and Mithun Santra and Carla Gon{\c c}alves and Michael Larsen and Marit Leuschner and Kim, {Yung Hae} and Joshua Brickman and Mark Bradley and Anne Grapin-Botton",
note = "Publisher Copyright: {\textcopyright} 2022 The Authors",
year = "2022",
doi = "10.1016/j.stemcr.2022.03.013",
language = "English",
volume = "17",
pages = "1215--1228",
journal = "Stem Cell Reports",
issn = "2213-6711",
publisher = "Cell Press",
number = "5",

}

RIS

TY - JOUR

T1 - Long-term feeder-free culture of human pancreatic progenitors on fibronectin or matrix-free polymer potentiates β cell differentiation

AU - Nakamura, Akiko

AU - Wong, Yan Fung

AU - Venturato, Andrea

AU - Michaut, Magali

AU - Venkateswaran, Seshasailam

AU - Santra, Mithun

AU - Gonçalves, Carla

AU - Larsen, Michael

AU - Leuschner, Marit

AU - Kim, Yung Hae

AU - Brickman, Joshua

AU - Bradley, Mark

AU - Grapin-Botton, Anne

N1 - Publisher Copyright: © 2022 The Authors

PY - 2022

Y1 - 2022

N2 - With the aim of producing β cells for replacement therapies to treat diabetes, several protocols have been developed to differentiate human pluripotent stem cells to β cells via pancreatic progenitors. While in vivo pancreatic progenitors expand throughout development, the in vitro protocols have been designed to make these cells progress as fast as possible to β cells. Here, we report on a protocol enabling a long-term expansion of human pancreatic progenitors in a defined medium on fibronectin, in the absence of feeder layers. Moreover, through a screening of a polymer library we identify a polymer that can replace fibronectin. Our experiments, comparing expanded progenitors to directly differentiated progenitors, show that the expanded progenitors differentiate more efficiently into glucose-responsive β cells and produce fewer glucagon-expressing cells. The ability to expand progenitors under defined conditions and cryopreserve them will provide flexibility in research and therapeutic production.

AB - With the aim of producing β cells for replacement therapies to treat diabetes, several protocols have been developed to differentiate human pluripotent stem cells to β cells via pancreatic progenitors. While in vivo pancreatic progenitors expand throughout development, the in vitro protocols have been designed to make these cells progress as fast as possible to β cells. Here, we report on a protocol enabling a long-term expansion of human pancreatic progenitors in a defined medium on fibronectin, in the absence of feeder layers. Moreover, through a screening of a polymer library we identify a polymer that can replace fibronectin. Our experiments, comparing expanded progenitors to directly differentiated progenitors, show that the expanded progenitors differentiate more efficiently into glucose-responsive β cells and produce fewer glucagon-expressing cells. The ability to expand progenitors under defined conditions and cryopreserve them will provide flexibility in research and therapeutic production.

KW - beta cells

KW - differentiation

KW - endocrine cells

KW - human

KW - long-term culture

KW - pancreas

KW - pancreatic progenitors

KW - screening

KW - transcriptome

U2 - 10.1016/j.stemcr.2022.03.013

DO - 10.1016/j.stemcr.2022.03.013

M3 - Journal article

C2 - 35452596

AN - SCOPUS:85129598401

VL - 17

SP - 1215

EP - 1228

JO - Stem Cell Reports

JF - Stem Cell Reports

SN - 2213-6711

IS - 5

ER -

ID: 307330469