Live cell microscopy of DNA damage response in Saccharomyces cerevisiae

Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review

Standard

Live cell microscopy of DNA damage response in Saccharomyces cerevisiae. / Pinela da Silva, Sonia Cristina; Gallina, Irene; Eckert-Boulet, Nadine Valerie; Lisby, Michael.

DNA repair protocols. Springer Science+Business Media, 2012. p. 433-443 (Methods in Molecular Biology, Vol. 920).

Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review

Harvard

Pinela da Silva, SC, Gallina, I, Eckert-Boulet, NV & Lisby, M 2012, Live cell microscopy of DNA damage response in Saccharomyces cerevisiae. in DNA repair protocols. Springer Science+Business Media, Methods in Molecular Biology, vol. 920, pp. 433-443. https://doi.org/10.1007/978-1-61779-998-3_30

APA

Pinela da Silva, S. C., Gallina, I., Eckert-Boulet, N. V., & Lisby, M. (2012). Live cell microscopy of DNA damage response in Saccharomyces cerevisiae. In DNA repair protocols (pp. 433-443). Springer Science+Business Media. Methods in Molecular Biology Vol. 920 https://doi.org/10.1007/978-1-61779-998-3_30

Vancouver

Pinela da Silva SC, Gallina I, Eckert-Boulet NV, Lisby M. Live cell microscopy of DNA damage response in Saccharomyces cerevisiae. In DNA repair protocols. Springer Science+Business Media. 2012. p. 433-443. (Methods in Molecular Biology, Vol. 920). https://doi.org/10.1007/978-1-61779-998-3_30

Author

Pinela da Silva, Sonia Cristina ; Gallina, Irene ; Eckert-Boulet, Nadine Valerie ; Lisby, Michael. / Live cell microscopy of DNA damage response in Saccharomyces cerevisiae. DNA repair protocols. Springer Science+Business Media, 2012. pp. 433-443 (Methods in Molecular Biology, Vol. 920).

Bibtex

@inbook{edc0257d2f374ab6bc821150ea3643ab,
title = "Live cell microscopy of DNA damage response in Saccharomyces cerevisiae",
abstract = "Fluorescence microscopy of the DNA damage response in living cells stands out from many other DNA repair assays by its ability to monitor the response to individual DNA lesions in single cells. This is particularly true in yeast, where the frequency of spontaneous DNA lesions is relatively low compared to organisms with much larger genomes such as mammalian cells. Single cell analysis of individual DNA lesions allows specific events in the DNA damage response to be correlated with cell morphology, cell cycle phase, and other specific characteristics of a particular cell. Moreover, fluorescence live cell imaging allows for multiple cellular markers to be monitored over several hours. This chapter reviews useful fluorescent markers and genotoxic agents for studying the DNA damage response in living cells and provides protocols for live cell imaging, time-lapse microscopy, and for induction of site-specific DNA lesions.",
author = "{Pinela da Silva}, {Sonia Cristina} and Irene Gallina and Eckert-Boulet, {Nadine Valerie} and Michael Lisby",
year = "2012",
doi = "10.1007/978-1-61779-998-3_30",
language = "English",
isbn = "978-1-61779-997-6",
series = "Methods in Molecular Biology",
publisher = "Springer Science+Business Media",
pages = "433--443",
booktitle = "DNA repair protocols",
address = "Singapore",

}

RIS

TY - CHAP

T1 - Live cell microscopy of DNA damage response in Saccharomyces cerevisiae

AU - Pinela da Silva, Sonia Cristina

AU - Gallina, Irene

AU - Eckert-Boulet, Nadine Valerie

AU - Lisby, Michael

PY - 2012

Y1 - 2012

N2 - Fluorescence microscopy of the DNA damage response in living cells stands out from many other DNA repair assays by its ability to monitor the response to individual DNA lesions in single cells. This is particularly true in yeast, where the frequency of spontaneous DNA lesions is relatively low compared to organisms with much larger genomes such as mammalian cells. Single cell analysis of individual DNA lesions allows specific events in the DNA damage response to be correlated with cell morphology, cell cycle phase, and other specific characteristics of a particular cell. Moreover, fluorescence live cell imaging allows for multiple cellular markers to be monitored over several hours. This chapter reviews useful fluorescent markers and genotoxic agents for studying the DNA damage response in living cells and provides protocols for live cell imaging, time-lapse microscopy, and for induction of site-specific DNA lesions.

AB - Fluorescence microscopy of the DNA damage response in living cells stands out from many other DNA repair assays by its ability to monitor the response to individual DNA lesions in single cells. This is particularly true in yeast, where the frequency of spontaneous DNA lesions is relatively low compared to organisms with much larger genomes such as mammalian cells. Single cell analysis of individual DNA lesions allows specific events in the DNA damage response to be correlated with cell morphology, cell cycle phase, and other specific characteristics of a particular cell. Moreover, fluorescence live cell imaging allows for multiple cellular markers to be monitored over several hours. This chapter reviews useful fluorescent markers and genotoxic agents for studying the DNA damage response in living cells and provides protocols for live cell imaging, time-lapse microscopy, and for induction of site-specific DNA lesions.

U2 - 10.1007/978-1-61779-998-3_30

DO - 10.1007/978-1-61779-998-3_30

M3 - Book chapter

C2 - 22941621

SN - 978-1-61779-997-6

T3 - Methods in Molecular Biology

SP - 433

EP - 443

BT - DNA repair protocols

PB - Springer Science+Business Media

ER -

ID: 45275708