Intron with transgenic marker (InTraM) facilitates high-throughput screening of endogenous gene reporter lines
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- Intron with transgenic marker (InTraM) facilitates high-throughput screening of endogenous gene reporter lines
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The generation and maintenance of genome edited zebrafish lines is typically labor intensive due to the lack of an easy visual read-out for the modification. To facilitate this process, we have developed a novel method that relies on the inclusion of an artificial intron with a transgenic marker (InTraM) within theknock-insequence of interest, which upon splicing produces a transcript with a precise and seamless modification. We have demonstrated this technology by replacing the stop codon of the zebrafishfli1agene with a transcriptional activator KALTA4, using an InTraM that enables red fluorescent protein expression in the heart.
Original language | English |
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Article number | 23391 |
Journal | Genesis |
Volume | 58 |
Issue number | 10-11 |
Number of pages | 5 |
ISSN | 1526-954X |
DOIs | |
Publication status | Published - 2020 |
- animal model, CRISPR, Cas9, genome editing, screening, transgenesis, zebrafish
Research areas
ID: 280117922